![Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Western-Blot-NBP2-01926-img0022.jpg "Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI2D5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.75 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 100-362 of human LHX1(NP_005559) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LHX1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 44.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.75 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for LIM1 Antibody (NBP2-01926)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunocytochemistry-NBP2-01926-img0024.jpg "Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.")
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0021.jpg "Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.")
![Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Flow-Cytometry-NBP2-01926-img0025.jpg "Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).")
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0012.jpg "Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0019.jpg "Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0014.jpg "Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0015.jpg "Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0020.jpg "Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.")
![Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Flow-Cytometry-NBP2-01926-img0023.jpg "Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM1 antibody, and then analyzed by flow cytometry.")
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![SDS-PAGE FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF-8_423-F8_332.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")