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Cerberus 1 Antibody [Unconjugated]

 (6 Publications)
 (1 Review)
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Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cerberus 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cerberus 1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cerberus 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1075) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1075
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1075-SP
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Cerberus 1 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cerberus 1
Thy18-Ala267
Accession # O95813
Specificity
Detects human Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human DAN, recombinant mouse Gremlin, and recombinant chicken Caronte is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CER1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1075 in the following application:
Publications
Read Publications using
AF1075 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cerberus 1 Antibody [Unconjugated]

  • CER1
  • cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily)
  • Cerberus 1
  • cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
  • cerberus
  • cerberus-related 1
  • Cerberus-related protein
  • DAN domain family member 4
  • DAND4
  • MGC119894
  • MGC119895
  • MGC96951

Background

Cerberus 1 functions during development by binding to and antagonizing some members of the bone morphogenetic protein (BMP) family. Cerberus 1 is alternatively known as cerberus-related 1 and is a homolog of Xenopus cerberus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cerberus 1 Antibody (AF1075)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Immunocytochemistry, Immunohistochemistry.


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Immunocytochemistry
(1)
Immunohistochemistry
(1)
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Human
(2)
Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF1075 Applications Species
Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M; Distinct pathways drive anterior hypoblast specification in the implanting human embryo Nature cell biology 2024-03-01 [PMID: 38443567] (Immunohistochemistry, Human, Mouse) Immunohistochemistry Human, Mouse
Karvas, RM;Zemke, JE;Ali, SS;Upton, E;Sane, E;Fischer, LA;Dong, C;Park, KM;Wang, F;Park, K;Hao, S;Chew, B;Meyer, B;Zhou, C;Dietmann, S;Theunissen, TW; 3D-cultured blastoids model human embryogenesis from pre-implantation to early gastrulation stages Cell stem cell 2023-09-07 [PMID: 37683602] (Immunocytochemistry, Human) Immunocytochemistry Human
Matteo A. Molè, Tim H. H. Coorens, Marta N. Shahbazi, Antonia Weberling, Bailey A. T. Weatherbee, Carlos W. Gantner et al. A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre Nature Communications 6/17/2021 [PMID: 34140473]
Mahaddalkar PU, Scheibner K, Pfluger S et al. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm Nature Biotechnology 9/1/2020 [PMID: 32341565]
Mahaddalkar PU, Scheibner K, Pfluger S et al. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm Nature Biotechnology 2020-09-01 [PMID: 32341565]
Hidefumi Iwashita, Nobuaki Shiraki, Daisuke Sakano, Takashi Ikegami, Masanobu Shiga, Kazuhiko Kume et al. Secreted Cerberus1 as a Marker for Quantification of Definitive Endoderm Differentiation of the Pluripotent Stem Cells PLoS ONE 5/22/2013 [PMID: 23717584]

Review for Cerberus 1 Antibody (AF1075) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1075:
Filter by Applications
ICC
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 ICC Mouse 01/05/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24176640
SpeciesMouse

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Recent Reviews

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 Verified Customer
01/05/2015
Application: ICC
Species: Mouse
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Bioinformatics

Gene Symbol CER1
Uniprot