![Knockout Validated: LDB1 Antibody [NBP1-31362] - Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/LDB1-Antibody-Western-Blot-NBP1-31362-img0015.jpg "Knockout Validated: LDB1 Antibody [NBP1-31362] - Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Ch, Pm, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LDB1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Porcine (98%), Chicken (97%), Xenopus (97%), Monkey (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LDB1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | LDB1 |
![Immunocytochemistry/Immunofluorescence: LDB1 Antibody [NBP1-31362] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 protein stained by LDB1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/LDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31362-img0014.jpg "Immunocytochemistry/Immunofluorescence: LDB1 Antibody [NBP1-31362] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 protein stained by LDB1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - mouse muscle.](http://images.novusbio.com/fullsize/LDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-31362-img0012.jpg "Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - mouse muscle.")
![Western Blot: LDB1 Antibody [NBP1-31362] - Sample: 1000 ug 293T whole cell lysate/extract A. 20 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgGC. Immunoprecipitation of LDB1 protein by 2. 5 ug of LDB1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/LDB1-Antibody-Western-Blot-NBP1-31362-img0006.jpg "Western Blot: LDB1 Antibody [NBP1-31362] - Sample: 1000 ug 293T whole cell lysate/extract A. 20 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgGC. Immunoprecipitation of LDB1 protein by 2. 5 ug of LDB1 antibody diluted at 1:1000.")
![Western Blot: LDB1 Antibody [NBP1-31362] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/LDB1-Antibody-Western-Blot-NBP1-31362-img0009.jpg "Western Blot: LDB1 Antibody [NBP1-31362] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - Paraffin-embedded U87 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/LDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-31362-img0005.jpg "Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - Paraffin-embedded U87 Xenograft, using antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - rat middle brain.](http://images.novusbio.com/fullsize/LDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-31362-img0011.jpg "Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - rat middle brain.")
![Western Blot: LDB1 Antibody [NBP1-31362] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (NBP1-31362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31362_rabbit-polyclonal-ldb1-antibody-91020242014659.jpg "Western Blot: LDB1 Antibody [NBP1-31362] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (NBP1-31362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")