![Western Blot: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Western-Blot-NBP2-71134-img0001.jpg "Western Blot: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI2D5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 100-362 of human LHX1(NP_005559) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LHX1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 44.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for LIM1 Antibody (NBP2-71134)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | LHX1 |
![Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71134-img0004.jpg "Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.")
![Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71134-img0009.jpg "Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.")
![Flow Cytometry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71134-img0003.jpg "Flow Cytometry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).")
![Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71134-img0005.jpg "Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.")
![Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71134-img0006.jpg "Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.")
![Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71134-img0007.jpg "Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.")
![Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71134-img0008.jpg "Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.")
![Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71134-img0010.jpg "Immunohistochemistry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.")
![Flow Cytometry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-LIM1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71134-img0002.jpg "Flow Cytometry: LIM1 Antibody (OTI2D5) - Azide and BSA Free [NBP2-71134] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-LIM1 antibody, and then analyzed by flow cytometry.")
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![Simple Western Pax6 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax6_AF8150_Simple_Western_17126.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")