![Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] - Immunochemical staining of human Methionine Aminopeptidase 2/METAP2 in human small intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Methionine-Aminopeptidase-2-METAP2-Antibody-Immunohistochemistry-Paraffin-NBP2-99115-img0004.jpg "Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] - Immunochemical staining of human Methionine Aminopeptidase 2/METAP2 in human small intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with purified, recombinant Human Methionine Aminopeptidase 2/METAP2 (Accession#: NP_006829.1; Met1-Tyr478) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | METAP2 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Methionine Aminopeptidase 2/METAP2 Antibody (NBP2-99115)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | METAP2 |
![Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: PC12 Whole Cell LysateLysates/proteins at 30 μg per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/antibody/nbp2-99115_rabbit-polyclonal-methionine-aminopeptidase-2-metap2-antibody-western-blot-3052024113920..jpg "Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: PC12 Whole Cell LysateLysates/proteins at 30 μg per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A:0.5 mg HepG2 Whole Cell Lysate4 µL anti-METAP2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-METAP2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 72 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99115_rabbit-polyclonal-methionine-aminopeptidase-2-metap2-antibody-immunoprecipitation-3052024114037..jpg "Immunoprecipitation: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] -Lane A:0.5 mg HepG2 Whole Cell Lysate4 µL anti-METAP2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-METAP2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 72 kDa")
![Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] - Lane A: METAP2 konckout Hela Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 10 μg per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99115_rabbit-polyclonal-methionine-aminopeptidase-2-metap2-antibody-western-blot-3052024114335..jpg "Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP2-99115] - Lane A: METAP2 konckout Hela Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 10 μg per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")