![Western Blot: MELK Antibody [NB100-782] - MELK in human testes lysate (RIPA buffer, 1.4E5 cells per lane) using this antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NB100-782-img0006.jpg "Western Blot: MELK Antibody [NB100-782] - MELK in human testes lysate (RIPA buffer, 1.4E5 cells per lane) using this antibody at 0.1 ug/ml.")
| Reactivity | Hu, Mu, Bv, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence KRLVEDILSSCKV corresponding to C-Terminus according to NP_055606.1. |
| Predicted Species | Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | MELK |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Experiments gave bands at approx. 75 kDa band and 150 kDa band in human testis lysates and in cell lines HeLa and Jurkat after 0.1 ug/ml antibody staining. The150 kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6 kDa band according to NP_055606.1. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MELK Antibody (NB100-782)Find related products by research area.
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| Gene Symbol | MELK |
| Entrez |
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| Uniprot |
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![Western Blot: MELK Antibody [NB100-782] - NB100-782 (0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Online Catalogue NAC1 to Quaking > Goat Anti-PPP1R15A Antibody](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NB100-782-img0001.jpg "Western Blot: MELK Antibody [NB100-782] - NB100-782 (0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Online Catalogue NAC1 to Quaking > Goat Anti-PPP1R15A Antibody")
![Western Blot: MELK Antibody [NB100-782] - NB 100-782 (0.5ug/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NB100-782-img0004.jpg "Western Blot: MELK Antibody [NB100-782] - NB 100-782 (0.5ug/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.")
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