![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (MLANA/788) - IHC-Prediluted [NBP2-48209] - Human Melanoma stained with Melan-A Monoclonal Antibody (MLANA/788).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-MLANA-788-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48209-img0003.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (MLANA/788) - IHC-Prediluted [NBP2-48209] - Human Melanoma stained with Melan-A Monoclonal Antibody (MLANA/788).")
| Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
| Applications | IHC |
| Clone | MLANA/788 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Recombinant human full-length Melan-A/MART-1 protein (Uniprot: Q16655) |
| Localization | Cytoplasmic |
| Marker | Melanoma Marker |
| Specificity | This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MLANA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. Use in Immunohistochemistry reported in scientific literature (PMID: 30606914). |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Melan-A/MART-1 Antibody (NBP2-48209)Find related products by research area.
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| Gene Symbol | MLANA |
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (MLANA/788) - IHC-Prediluted [NBP2-48209] - Human Testis stained with Melan-A Monoclonal Antibody (MLANA/788).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-MLANA-788-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48209-img0002.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (MLANA/788) - IHC-Prediluted [NBP2-48209] - Human Testis stained with Melan-A Monoclonal Antibody (MLANA/788).")
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