Home » Melan-A/MART-1 » Melan-A/MART-1 Antibodies » Melan-A/MART-1 Antibody (M2-9E3) [Janelia Fluor® 549]
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Melan-A/MART-1 Antibody (M2-9E3) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
M2-9E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-33150AF350
Alexa Fluor 405
NBP2-33150AF405
Alexa Fluor 488
NBP2-33150AF488
Alexa Fluor 532
NBP2-33150AF532
Alexa Fluor 594
NBP2-33150AF594
Alexa Fluor 647
NBP2-33150AF647
Alexa Fluor 700
NBP2-33150AF700
Alexa Fluor 750
NBP2-33150AF750
Allophycocyanin
NBP2-33150APC
Biotin
NBP2-33150B
DyLight 350
NBP2-33150UV
DyLight 405
NBP2-33150V
DyLight 488
NBP2-33150G
DyLight 550
NBP2-33150R
DyLight 594
NBP2-33150DL594
DyLight 650
NBP2-33150C
DyLight 680
NBP2-33150FR
DyLight 755
NBP2-33150IR
FITC
NBP2-33150F
HRP
NBP2-33150H
Janelia Fluor 646
NBP2-33150JF646
PE
NBP2-33150PE
PerCP
NBP2-33150PCP
Unconjugated
NBP2-15198-0.1mg
mFluor Violet 450 SE
NBP2-33150MFV450
mFluor Violet 500 SE
NBP2-33150MFV500
mFluor Violet 610 SE
NBP2-33150MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-15198-0.1mg
Azide and BSA Free
NBP2-33150
IHC-Prediluted
NBP2-44388
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Melan-A/MART-1 Antibody (M2-9E3) [Janelia Fluor® 549] Summary

Immunogen
Recombinant Melan-A/MART-1 protein (Uniprot: Q16655)
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLANA
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Melan-A/MART-1 Antibody (M2-9E3) [Janelia Fluor® 549]

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • Melan-A
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Background

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MLANA