![Western Blot: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150] - Western Blot Analysis of COLO38 cell lysate using Melan-A/MART-1 Antibody (M2-9E3).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-9E3-Azide-and-BSA-Free-Western-Blot-NBP2-33150-img0003.jpg "Western Blot: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150] - Western Blot Analysis of COLO38 cell lysate using Melan-A/MART-1 Antibody (M2-9E3).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | M2-9E3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15198). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant Melan-A/MART-1 protein (Uniprot: Q16655) |
| Localization | Cytoplasmic |
| Marker | Melanoma Marker |
| Specificity | This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MLANA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Melan-A/MART-1 Antibody (NBP2-33150)Find related products by research area.
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![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33150-img0002.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33150-img0001.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - Azide and BSA Free [NBP2-33150] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.")
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