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Mcl-1 Antibody (OTI10F6) [Allophycocyanin/Cy7]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
OTI10F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72598AF350
Alexa Fluor 405
NBP2-72598AF405
Alexa Fluor 488
NBP2-72598AF488
Alexa Fluor 532
NBP2-72598AF532
Alexa Fluor 594
NBP2-72598AF594
Alexa Fluor 647
NBP2-72598AF647
Alexa Fluor 700
NBP2-72598AF700
Alexa Fluor 750
NBP2-72598AF750
Allophycocyanin
NBP2-72598APC
Biotin
NBP2-72598B
CoraFluor 1
NBP2-72598CL1
DyLight 350
NBP2-72598UV
DyLight 405
NBP2-72598V
DyLight 488
NBP2-72598G
DyLight 550
NBP2-72598R
DyLight 594
NBP2-72598DL594
DyLight 650
NBP2-72598C
DyLight 680
NBP2-72598FR
DyLight 755
NBP2-72598IR
FITC
NBP2-72598F
HRP
NBP2-72598H
Janelia Fluor 525
NBP2-72598JF525
Janelia Fluor 549
NBP2-72598JF549
Janelia Fluor 585
NBP2-72598JF585
Janelia Fluor 635
NBP2-72598JF635
Janelia Fluor 646
NBP2-72598JF646
Janelia Fluor 669
NBP2-72598JF669
PE
NBP2-72598PE
PE/Atto594
NBP2-72598PEATT594
PE/Cy5.5
NBP2-72598PECY55
PE/Cy7
NBP2-72598PECY7
PerCP
NBP2-72598PCP
Unconjugated
NBP2-45773
mFluor Violet 450 SE
NBP2-72598MFV450
mFluor Violet 500 SE
NBP2-72598MFV500
mFluor Violet 610 SE
NBP2-72598MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45773
Azide and BSA Free
NBP2-72598
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Mcl-1 Antibody (OTI10F6) [Allophycocyanin/Cy7] Summary

Immunogen
Full length recombinant protein of human Mcl-1 (NP_068779) produced in HEK293T cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MCL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Mcl-1 Antibody (OTI10F6) [Allophycocyanin/Cy7]

  • BCL2L3
  • bcl2-L-3
  • BCL2L3MGC104264
  • Bcl-2-like protein 3
  • Bcl-2-related protein EAT/mcl1
  • EAT
  • induced myeloid leukemia cell differentiation protein Mcl-1
  • Mcl1
  • Mcl-1
  • mcl1/EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • MGC1839
  • myeloid cell leukemia ES
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • TM

Background

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-72598APCCY7
Mcl-1 Antibody (OTI10F6) [Allophycocyanin/Cy7]
Species: Hu
Applications: Flow, ICC/IF, IHC

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MCL1