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Mcl-1 Antibody (OTI10F6) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI10F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72598AF350
Alexa Fluor 405
NBP2-72598AF405
Alexa Fluor 488
NBP2-72598AF488
Alexa Fluor 532
NBP2-72598AF532
Alexa Fluor 594
NBP2-72598AF594
Alexa Fluor 647
NBP2-72598AF647
Alexa Fluor 700
NBP2-72598AF700
Alexa Fluor 750
NBP2-72598AF750
Allophycocyanin
NBP2-72598APC
Allophycocyanin/Cy7
NBP2-72598APCCY7
Biotin
NBP2-72598B
CoraFluor 1
NBP2-72598CL1
DyLight 350
NBP2-72598UV
DyLight 405
NBP2-72598V
DyLight 488
NBP2-72598G
DyLight 550
NBP2-72598R
DyLight 594
NBP2-72598DL594
DyLight 650
NBP2-72598C
DyLight 680
NBP2-72598FR
DyLight 755
NBP2-72598IR
FITC
NBP2-72598F
HRP
NBP2-72598H
Janelia Fluor 549
NBP2-72598JF549
Janelia Fluor 585
NBP2-72598JF585
Janelia Fluor 635
NBP2-72598JF635
Janelia Fluor 646
NBP2-72598JF646
Janelia Fluor 669
NBP2-72598JF669
PE
NBP2-72598PE
PE/Atto594
NBP2-72598PEATT594
PE/Cy5.5
NBP2-72598PECY55
PE/Cy7
NBP2-72598PECY7
PerCP
NBP2-72598PCP
Unconjugated
NBP2-45773
mFluor Violet 450 SE
NBP2-72598MFV450
mFluor Violet 500 SE
NBP2-72598MFV500
mFluor Violet 610 SE
NBP2-72598MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45773
Azide and BSA Free
NBP2-72598
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Mcl-1 Antibody (OTI10F6) [Janelia Fluor® 525] Summary

Immunogen
Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MCL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Mcl-1 Antibody (OTI10F6) [Janelia Fluor® 525]

  • BCL2L3
  • bcl2-L-3
  • BCL2L3MGC104264
  • Bcl-2-like protein 3
  • Bcl-2-related protein EAT/mcl1
  • EAT
  • induced myeloid leukemia cell differentiation protein Mcl-1
  • Mcl1
  • Mcl-1
  • mcl1/EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • MGC1839
  • myeloid cell leukemia ES
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • TM

Background

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Mcl-1 Antibody
(OTI10F6) [Janelia Fl ...
NBP2-72598JF525
Mcl-1 Antibody (OTI10F6) [Janelia Fluor® 525]
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

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Bioinformatics

Gene Symbol MCL1