Home » Mcl-1 » Mcl-1 Antibodies » Mcl-1 Antibody (OTI10F6)
Novus Biologicals products are now on bio-techne.com

Mcl-1 Antibody (OTI10F6)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.
Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Carcinoma of prostate tissue.
Flow Cytometry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of HEK293T cells transfected with either pCMV6-ENTRY MCL1.
Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of extracts (10ug).
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of breast tissue.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Kidney tissue within the normal limits.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Carcinoma of liver tissue.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Carcinoma of pancreas tissue.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of prostate tissue.
Immunofluorescence: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MCL-1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI10F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72598AF350
Alexa Fluor 405
NBP2-72598AF405
Alexa Fluor 488
NBP2-72598AF488
Alexa Fluor 532
NBP2-72598AF532
Alexa Fluor 594
NBP2-72598AF594
Alexa Fluor 647
NBP2-72598AF647
Alexa Fluor 700
NBP2-72598AF700
Alexa Fluor 750
NBP2-72598AF750
Allophycocyanin
NBP2-72598APC
Allophycocyanin/Cy7
NBP2-72598APCCY7
Biotin
NBP2-72598B
CoraFluor 1
NBP2-72598CL1
DyLight 350
NBP2-72598UV
DyLight 405
NBP2-72598V
DyLight 488
NBP2-72598G
DyLight 550
NBP2-72598R
DyLight 594
NBP2-72598DL594
DyLight 650
NBP2-72598C
DyLight 680
NBP2-72598FR
DyLight 755
NBP2-72598IR
FITC
NBP2-72598F
HRP
NBP2-72598H
Janelia Fluor 525
NBP2-72598JF525
Janelia Fluor 549
NBP2-72598JF549
Janelia Fluor 585
NBP2-72598JF585
Janelia Fluor 635
NBP2-72598JF635
Janelia Fluor 646
NBP2-72598JF646
Janelia Fluor 669
NBP2-72598JF669
PE
NBP2-72598PE
PE/Atto594
NBP2-72598PEATT594
PE/Cy5.5
NBP2-72598PECY55
PE/Cy7
NBP2-72598PECY7
PerCP
NBP2-72598PCP
mFluor Violet 450 SE
NBP2-72598MFV450
mFluor Violet 500 SE
NBP2-72598MFV500
mFluor Violet 610 SE
NBP2-72598MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72598
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Mcl-1 Antibody (OTI10F6) Summary

Immunogen
Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MCL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:200 - 1:1000
Theoretical MW
37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mcl-1 Antibody (OTI10F6)

  • BCL2L3
  • bcl2-L-3
  • BCL2L3MGC104264
  • Bcl-2-like protein 3
  • Bcl-2-related protein EAT/mcl1
  • EAT
  • induced myeloid leukemia cell differentiation protein Mcl-1
  • Mcl1
  • Mcl-1
  • mcl1/EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • MGC1839
  • myeloid cell leukemia ES
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • TM

Background

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Bcl-xL Antibody
NB100-56104
Western Blot Bcl-xL Antibody
Immunohistochemistry-Paraffin Bcl-xL Antibody
Western Blot Bcl-xL Antibody
(1 Review)
(3 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
Bcl-2 Antibody
NB100-56098
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
(2 Reviews)
(26 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Bax Antibody (6A7)
NBP1-28566
Western Blot Bax Antibody (6A7)
Western Blot Bax Antibody (6A7)
Immunohistochemistry-Frozen Bax Antibody (6A7)
(3 Reviews)
(20 Publications)
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
BAK Antibody
[Unconjugated]
AF816
Western Blot BAK Antibody [Unconjugated]
Immunocytochemistry BAK Antibody [Unconjugated]
Immunohistochemistry BAK Antibody [Unconjugated]
(5 Publications)
Species: Hu
Applications: ICC, IHC, WB
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(384 Publications)
Species: Hu, Mu
Applications: ICC, IHC
Bim Antibody - BSA
Free
NBP1-76963
Immunocytochemistry/ Immunofluorescence Bim Antibody - BSA Free
Western Blot Bim Antibody - BSA Free
Western Blot Bim Antibody - BSA Free
(8 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, Simple Western, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Noxa Antibody
(114C307.1) - Non-Rec ...
NB600-1159
Immunocytochemistry/ Immunofluorescence Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal
Immunohistochemistry-Paraffin Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal
Flow Cytometry Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal
(2 Reviews)
(76 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
XIAP Antibody
[Unconjugated]
AF8221
Western Blot XIAP Antibody [Unconjugated]
Simple Western XIAP Antibody [Unconjugated]
Knockout Validated XIAP Antibody [Unconjugated]
(23 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
Cytochrome c
Antibody (7H8.2C12) - ...
NB100-56503
Flow Cytometry Cytochrome c Antibody (7H8.2C12) - BSA Free
Flow (Intracellular) Cytochrome c Antibody (7H8.2C12) - BSA Free
Western Blot Cytochrome c Antibody (7H8.2C12) - BSA Free
(61 Publications)
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
STAT3 Antibody
(232209) [Unconjugate
MAB1799
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
Western Blot STAT3 Antibody (232209) [Unconjugated]
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
(22 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
BID Antibody
[Unconjugated]
AF860
Simple Western BID Antibody [Unconjugated]
Western Blot BID Antibody [Unconjugated]
Western Blot BID Antibody [Unconjugated]
(1 Review)
(38 Publications)
Species: Hu, Mu
Applications: IP, Simple Western, WB
TRAIL/TNFSF10
[Unconjugated]
375-TL
TRAIL/TNFSF10 [Unconjugated]
(1 Review)
(54 Publications)
Species: Hu
Applications: BA
PARP Antibody
(194C1439) - Cleaved ...
NBP2-27335
Immunocytochemistry/ Immunofluorescence PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
Western Blot PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
Western Blot PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
(1 Review)
(9 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin p21/CIP1/CDKN1A Antibody (WA-1)
(24 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(807 Publications)
Species: Hu
Applications: BA

Publications for Mcl-1 Antibody (NBP2-45773) (0)

There are no publications for Mcl-1 Antibody (NBP2-45773).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Mcl-1 Antibody (NBP2-45773) (0)

There are no reviews for Mcl-1 Antibody (NBP2-45773). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mcl-1 Antibody (NBP2-45773) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Mcl-1 Products

Research Areas for Mcl-1 Antibody (NBP2-45773)

Find related products by research area.

Blogs on Mcl-1.

Check out the latest blog posts on Mcl-1.

  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Mcl-1 Antibody (OTI10F6) and receive a gift card or discount.


Bioinformatics

Gene Symbol MCL1