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MAGEA4 Antibody

Images

 
Western Blot: MAGEA4 Antibody [NBP2-17204] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody [NBP2-17204] - MAGEA4 antibody detects MAGEA4 protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4 paraformaldehyde at RT for ...read more
Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP2-17204] - Human breast cancer, using MAGEA4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MAGEA4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MAGEA4. The exact sequence is proprietary.
Predicted Species
Rhesus Macaque (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAGEA4
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for MAGEA4 Antibody

  • CT1.4
  • MAGE-41
  • MAGE4A
  • MAGE4B
  • MAGEA4
  • MAGE-X2 antigen
  • MAGE-X2
  • melanoma antigen family A, 4
  • melanoma-associated antigen 4
  • member 4

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAGEA4
Uniprot