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MAGEA12 Antibody (3A10)

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Western Blot: MAGEA12 Antibody (3A10) [H00004111-M01] - MAGEA12 monoclonal antibody (M01), clone 3A10. Analysis of MAGEA12 expression in human liver.
Western Blot: MAGEA12 Antibody (3A10) [H00004111-M01] - MAGEA12 monoclonal antibody (M01), clone 3A10. Analysis of MAGEA12 expression in Raw 264.7.
Western Blot: MAGEA12 Antibody (3A10) [H00004111-M01] - MAGEA12 monoclonal antibody (M01), clone 3A10. Analysis of MAGEA12 expression in Jurkat.
Western Blot: MAGEA12 Antibody (3A10) [H00004111-M01] - MAGEA12 monoclonal antibody (M01), clone 3A10. Analysis of MAGEA12 expression in NIH/3T3.
ELISA: MAGEA12 Antibody (3A10) [H00004111-M01] - Detection limit for recombinant GST tagged MAGEA12 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
3A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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MAGEA12 Antibody (3A10) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MAGEA12 (NP_005358, 70 a.a. ~ 168 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLPTTINYTLWSQSDEGSSNEEQEGPSTFPDLETSFQVALSRKMAELVHFLLLKYRAREPFTKAEMLGSVIRNFQDFFPVIFSKASEYLQLVFGIEVVE
Specificity
MAGEA12 - melanoma antigen family A, 12 (3A10)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAGEA12
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAGEA12 Antibody (3A10)

  • Cancer/testis antigen 1.12
  • CT1.12MAGE12F antigen
  • MAGE-12 antigen
  • MAGE12cancer/testis antigen family 1, member 12
  • melanoma antigen family A, 12
  • melanoma-associated antigen 12

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAGEA12
Entrez
Uniprot