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MAGEA4 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP1-84365] - Analysis in human testis and endometrium tissues using NBP1-84365 antibody. Corresponding MAGEA4 RNA-seq data are presented for ...read more
Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody [NBP1-84365] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP1-84365] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP1-84365] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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MAGEA4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: WRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPASNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAGEA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MAGEA4 Protein (NBP1-84365PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAGEA4 Antibody

  • CT1.4
  • MAGE-41
  • MAGE4A
  • MAGE4B
  • MAGEA4
  • MAGE-X2 antigen
  • MAGE-X2
  • melanoma antigen family A, 4
  • melanoma-associated antigen 4
  • member 4

Background

MAGEA4 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAGEA4
Entrez
Uniprot