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LSD1 Activity/Inhibition Assay Kit (Colorimetric)

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LSD1 Assay/Inhibitor Screening Assay Kit [KA1525] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
LSD1 Assay/Inhibitor Screening Assay Kit [KA1525] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func
Suitable Sample Type
Nuclear extracts; purified LSD1 enzymes
Standard Curve Range
0.5 to 20 ug nuclear extract; 5 to 500 ng purified enzyme
Sensitivity
0.5 ug nuclear extract; 5 ng purified enzyme

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LSD1 Activity/Inhibition Assay Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve LSD1 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for screening LSD1 demethylase inhibitors.
Standard Curve Range
0.5 to 20 ug nuclear extract; 5 to 500 ng purified enzyme
Sensitivity
0.5 ug nuclear extract; 5 ng purified enzyme
Assay Type
Colorimetric
Sample Volume
1- 4 uL
Kit Type
Activity/Inhibition Assay Kit (Colorimetric)
Gene
KDM1A

Applications/Dilutions

Dilutions
  • Functional
Publications
Read Publication using KA1525.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 8-Well Assay Strips (With Frame)
  2. Adhesive Covering Film
  3. LD1 (10X Wash Buffer)
  4. LD2 (LSD1 Assay Buffer)
  5. LD3 (LSD1 Substrate, 50 µg/mL)
  6. LD4 (LSD1 Assay Standard, 50 µg/mL)
  7. LD5 (Capture Antibody, 1000 µg/mL)
  8. LD6 (Detection Antibody, 400 µg/mL)
  9. LD7 (LSD1 Inhibitor Tranylcypromine, 1 mM)
  10. LD8 (Developer Solution)
  11. LD9 (Stop Solution)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LSD1 Activity/Inhibition Assay Kit (Colorimetric)

  • amine oxidase (flavin containing) domain 2
  • AOF2
  • AOF2lysine-specific histone demethylase 1
  • BHC110
  • BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
  • Flavin-containing amine oxidase domain-containing protein 2
  • KIAA0601KDM1
  • LSD1
  • LSD1BRAF35-HDAC complex protein BHC110
  • lysine (K)-specific demethylase 1
  • Lysine (K)specific Demethylase 1A
  • Lysine (K)-specific Demethylase 1A
  • lysine-specific histone demethylase 1A

Background

LSD1 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for screening LSD1 demethylase inhibitors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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  LSD1 Assay/Inhibitor Screening Assay Kit
  PDF Datasheet
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Bioinformatics

Gene Symbol KDM1A