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RCOR1/CoREST Antibody (9B3R7)

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Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of extracts of C2C12 cells, using RCOR1/CoREST Rabbit mAb (NBP3-16225) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry-Paraffin: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Mouse brain using RCOR1/CoREST Rabbit mAb (NBP3-16225) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA ...read more
Chromatin Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RCOR1/CoREST antibody (NBP3-16225) and rabbit IgG.The amount of ...read more
Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of extracts of various cell lines, using RCOR1/CoREST Rabbit mAb (NBP3-16225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunohistochemistry-Paraffin: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Rat lung using RCOR1/CoREST Rabbit mAb (NBP3-16225) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA ...read more
Immunohistochemistry-Paraffin: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Human appendix using RCOR1/CoREST Rabbit mAb (NBP3-16225) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM ...read more
Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of 300ug extracts of THP-1 cells using 3ug RCOR1/CoREST antibody (NBP3-16225). Western blot was performed from the immunoprecipitate using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP, ChIP
Clone
9B3R7
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RCOR1/CoREST Antibody (9B3R7) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RCOR1/CoREST (Q9UKL0). MPAMVEKGPEVSGKRRGRNNAAASASAAAASAAASAACASPAATAASGAAASSASAAAASAAAAPNNGQNKSLAAAAPNGNSSSNSWEEGSSGSSSDEEH
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RCOR1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5ug antibody for 10ug-15ug of Chromatin
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RCOR1/CoREST Antibody (9B3R7)

  • CoREST
  • KIAA0071CORESTRCORREST corepressor
  • Protein CoREST
  • RCOR1
  • RCOR1/CoREST
  • REST corepressor 1

Background

Brain type II sodium channels are important in neuronal phenotyping and are only seen at high levels in neurons. Studies have shown that CoREST functions as a corepressor for REST. This interaction between REST and CoREST is accomplished by means of a single zinc finger binding motif originating from REST. Together, the REST-CoREST complex mediates long term repression of the type II sodium channel gene (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RCOR1