![Western Blot: AOF1 Antibody (5E10C1) [NBP2-52415] - Western blot aAagainst human AOF1 (AA: 6-129) recombinant protein. (Expected MW is 40 kDa)](http://images.novusbio.com/fullsize/AOF1-Antibody-5E10C1-Western-Blot-NBP2-52415-img0005.jpg "Western Blot: AOF1 Antibody (5E10C1) [NBP2-52415] - Western blot aAagainst human AOF1 (AA: 6-129) recombinant protein. (Expected MW is 40 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 5E10C1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human AOF1 (AA: 6-129) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KDM1B |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 92 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Western Blot DEK Antibody (715524) [Unconjugated]](https://images.novusbio.com/images2/DEK_MAB7020_Western_Blot_11326.jpg)
![Immunohistochemistry DEK Antibody (715524) [Unconjugated]](https://images.novusbio.com/images2/DEK_MAB7020_Immunohistochemistry_20290.jpg)
![Western Blot SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Western_Blot_5161.jpg)
![Chromatin Immunoprecipitation (ChIP) SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Chromatin_Immunoprecipitation_11224.jpg)
![Simple Western SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Simple_Western_16676.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KDM1B |
![Immunocytochemistry/Immunofluorescence: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of HeLa cells using AOF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/AOF1-Antibody-5E10C1-Immunofluorescence-NBP2-52415-img0002.jpg "Immunocytochemistry/Immunofluorescence: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of HeLa cells using AOF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.")
![Immunohistochemistry: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of paraffin-embedded rectum cancer tissues using AOF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AOF1-Antibody-5E10C1-Immunohistochemistry-NBP2-52415-img0007.jpg "Immunohistochemistry: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of paraffin-embedded rectum cancer tissues using AOF1 mouse mAb with DAB staining.")
![Flow Cytometry: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of HeLa cells using AOF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/AOF1-Antibody-5E10C1-Flow-Cytometry-NBP2-52415-img0010.jpg "Flow Cytometry: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of HeLa cells using AOF1 mouse mAb (green) and negative control (red).")
![Western Blot: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis using AOF1 mAb against HEK293 (1) and AOF1 (AA: 6-129)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AOF1-Antibody-5E10C1-Western-Blot-NBP2-52415-img0006.jpg "Western Blot: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis using AOF1 mAb against HEK293 (1) and AOF1 (AA: 6-129)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: AOF1 Antibody (5E10C1) [NBP2-52415] - Immunofluorescence analysis of HeLa cells using AOF1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/AOF1-Antibody-5E10C1-Immunofluorescence-NBP2-52415-img0001.jpg "Immunocytochemistry/Immunofluorescence: AOF1 Antibody (5E10C1) [NBP2-52415] - Immunofluorescence analysis of HeLa cells using AOF1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.")
![Immunohistochemistry: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of paraffin-embedded esophageal cancer tissues using AOF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AOF1-Antibody-5E10C1-Immunohistochemistry-NBP2-52415-img0003.jpg "Immunohistochemistry: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of paraffin-embedded esophageal cancer tissues using AOF1 mouse mAb with DAB staining.")
![ELISA: AOF1 Antibody (5E10C1) [NBP2-52415] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/AOF1-Antibody-5E10C1-ELISA-NBP2-52415-img0011.jpg "ELISA: AOF1 Antibody (5E10C1) [NBP2-52415] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).")
![Western Blot G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241252118.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-412024125214.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241251265.jpg)
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
![Immunocytochemistry Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Immunocytochemistry_7093.jpg)
![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
![Chromatin Immunoprecipitation (ChIP) Nanog Antibody [Unconjugated]](https://images.novusbio.com/images2/Nanog_AF1997_Chromatin_Immunoprecipitation_11222.jpg)
![Immunocytochemistry Nanog Antibody [Unconjugated]](https://images.novusbio.com/images2/Nanog_AF1997_Immunocytochemistry_6478.jpg)
![Immunocytochemistry Nanog Antibody [Unconjugated]](https://images.novusbio.com/images2/Nanog_AF1997_Immunocytochemistry_6636.jpg)
![Knockout Validated STAT3 Antibody (232209) [Unconjugated]](https://images.novusbio.com/images2/STAT3_MAB1799_Simple_Western_21000.jpg)
![Western Blot STAT3 Antibody (232209) [Unconjugated]](https://images.novusbio.com/images2/STAT3_MAB1799_Western_Blot_5993.jpg)
![Knockout Validated STAT3 Antibody (232209) [Unconjugated]](https://images.novusbio.com/images2/STAT3_MAB1799_Knockout_Validated_21531.jpg)
![Simple Western c-Rel Antibody [Unconjugated]](https://images.novusbio.com/images2/cRel_AF2699_Simple_Western_16940.jpg)
![Western Blot c-Rel Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2699_human-mouse-c-rel-affinity-purified-polyclonal-ab-western-blot-2102024121216..jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")