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LSD1 Antibody [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 635

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76688AF350
Alexa Fluor 405
NBP1-76688AF405
Alexa Fluor 488
NBP1-76688AF488
Alexa Fluor 532
NBP1-76688AF532
Alexa Fluor 594
NBP1-76688AF594
Alexa Fluor 647
NBP1-76688AF647
Alexa Fluor 700
NBP1-76688AF700
Alexa Fluor 750
NBP1-76688AF750
Biotin
NBP1-76688B
CoraFluor 1
NBP1-76688CL1
DyLight 350
NBP1-76688UV
DyLight 405
NBP1-76688V
DyLight 488
NBP1-76688G
DyLight 550
NBP1-76688R
DyLight 594
NBP1-76688DL594
DyLight 650
NBP1-76688C
DyLight 680
NBP1-76688FR
DyLight 755
NBP1-76688IR
FITC
NBP1-76688F
HRP
NBP1-76688H
Janelia Fluor 525
NBP1-76688JF525
Janelia Fluor 549
NBP1-76688JF549
Janelia Fluor 585
NBP1-76688JF585
Janelia Fluor 646
NBP1-76688JF646
Janelia Fluor 669
NBP1-76688JF669
PerCP
NBP1-76688PCP
Unconjugated
NBP1-76688
mFluor Violet 450 SE
NBP1-76688MFV450
mFluor Violet 500 SE
NBP1-76688MFV500
mFluor Violet 610 SE
NBP1-76688MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-76688
BSA Free
NBP1-76688
BSA Free
NBP1-76688-0.025mg
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LSD1 Antibody [Janelia Fluor® 635] Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the center of human LSD1. The immunogen is located within amino acids 140 - 190 of LSD1. Amino Acid Squence: QAPPEEENESEPEEPS
Marker
Nucleus Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM1A
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for LSD1 Antibody [Janelia Fluor® 635]

  • amine oxidase (flavin containing) domain 2
  • AOF2
  • AOF2lysine-specific histone demethylase 1
  • BHC110
  • BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
  • Flavin-containing amine oxidase domain-containing protein 2
  • KIAA0601KDM1
  • LSD1
  • LSD1BRAF35-HDAC complex protein BHC110
  • lysine (K)-specific demethylase 1
  • Lysine (K)specific Demethylase 1A
  • Lysine (K)-specific Demethylase 1A
  • lysine-specific histone demethylase 1A

Background

LSD1 a recently identified, first known lysine-specific histone demethylase, is an 886 amino acid nuclear protein belonging to flavin monoamine oxidase family. It contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. This protein is ubiquitously expressed and is a component of several histone deacetylase complexes. LSD1 acts as a component of the CoREST and other transcriptional co-repressor complexes and also plays an important role in silencing neuronal-specific genes in non-neuronal cells. It is also known to demethylate Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation. Reports suggest that it plays an important role in stimulating androgen-receptor-dependent transcription converting oxygen to hydrogen peroxide (might use alternative electron acceptors). Along with nuclear FHL2 it serves as a novel biomarker predictive for prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KDM1A