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LOX Antibody

Images

 
Western Blot: LOX Antibody [NBP2-24877] - Analysis of mouse ear lysate when LOX antibody (NBP2-24877) was used at 1:1000. The band shown is the expected inactive 50 kD proenzyme.
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NBP2-24877] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated ...read more
Immunohistochemistry-Paraffin: LOX Antibody [NBP2-24877] - Analysis of LOX in formalin-fixed, paraffin-embedded mouse skin using an isotype control (top) and NBP2-24877 (bottom) at 1:100.
Western Blot: LOX Antibody [NBP2-24877] - Analysis of LOX in A) human skin and B) mouse ear skin lysate using NBP2-24877 at 1:500. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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LOX Antibody Summary

Immunogen
A portion of amino acids 305-338 of human LOX protein were used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LOX
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry reported in scientific literature (PMID 29404848)
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated reported in scientific literature (PMID 31173582)
  • Western Blot 1:100-1:2000
Publications
Read Publications using
NBP2-24877 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 26224524). Sheep reactivity reported in scientific literature (PMID: 26563105).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.1 ml crude whole Rabbit sera with 50% Glycerol
Preservative
0.025% Sodium Azide
Purity
Unpurified

Alternate Names for LOX Antibody

  • AAT10
  • Lysyl Oxidase/LOX
  • lysyl oxidaseEC 1.4.3.13
  • MGC105112
  • protein-lysine 6-oxidase

Background

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development; and, its aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LOX Antibody (NBP2-24877)(22)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Sheep.

We have publications tested in 5 applications: IF/IHC, IHC-Fr, IHC-P, Knockout Validated, WB.


Filter By Application
IF/IHC
(2)
IHC-Fr
(3)
IHC-P
(6)
Knockout Validated
(1)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
Rat
(2)
Porcine
(4)
Sheep
(2)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NBP2-24877 Applications Species
Wang C Regulatory Roles of Fibril-Forming Collagens in Cartilaginous Tissue Biomechanics and Mechanobiology Thesis 2023-01-01
Fuqua JD, Lawrence MM, Hettinger ZR et al. Impaired proteostatic mechanisms other than decreased protein synthesis limit old skeletal muscle recovery after disuse atrophy Journal of cachexia, sarcopenia and muscle 2023-07-14 [PMID: 37448295] (WB, Rat) WB Rat
Abbott C, Lawrence M, Kobak K et al. A novel stable isotope approach demonstrates surprising degree of age-related decline in skeletal muscle collagen proteostasis Function 2021-05-10 [PMID: 34124684] (WB, Mouse) WB Mouse
Wang C, Brisson BK, Terajima M et al. Type III Collagen is a Key Regulator of the Collagen Fibrillar Structure and Biomechanics of Articular Cartilage and Meniscus Matrix Biol. 2019-10-23 [PMID: 31655293] (WB, Mouse) WB Mouse
Terajima M, Taga Y, Cabral WA et al. Cyclophilin B control of lysine post-translational modifications of skin type I collagen PLoS Genet. 2019-06-01 [PMID: 31173582] (WB, IF/IHC, Knockout Validated, Mouse) WB, IF/IHC, Knockout Validated Mouse
Rossow L, Veitl S, Vorlova S et al LOX-catalyzed collagen stabilization is a proximal cause for intrinsic resistance to chemotherapy. Oncogene. 2018-09-01 [PMID: 29780168] (Human)

Details:
Citation using the HRP version of this antibody.
Human
Connell PS, Vekilov DP, Diaz CM et al. Eliminating Regurgitation Reduces Fibrotic Remodeling of Functional Mitral Regurgitation Conditioned Valves. Ann Biomed Eng 2018-02-05 [PMID: 29404848] (IF/IHC, Porcine) IF/IHC Porcine
Smith LR, Hammers DW, Sweeney HL, Barton ER. Increased collagen cross-linking is a signature of dystrophin-deficient muscle. Muscle Nerve. 2015-11-30 [PMID: 26616495] (WB, Mouse) WB Mouse
Stephens EH, Nguyen TC, Blazejewski JG et al. Extracellular matrix remodeling in wound healing of critical size defects in the mitral valve leaflet. Heart Vessels. 2015-11-12 [PMID: 26563105] (IHC-P, Sheep) IHC-P Sheep
Connell PS, Azimuddin AF, Kim SE et al. Regurgitation Hemodynamics Alone Cause Mitral Valve Remodeling Characteristic of Clinical Disease States In Vitro. Ann Biomed Eng. 2015-07-30 [PMID: 26224524] (IHC-P, Porcine)

Details:
LOX antibody (Imgenex: IMG-6442A) was used for IHC-P analysis of lysyl oxidase protein in experiments involving organ cultures of mitral valves that were obtained from adolescent (6–9 months) porcine animals.
IHC-P Porcine
Show All 22 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional LOX Products

Research Areas for LOX Antibody (NBP2-24877)

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Blogs on LOX.

LOX: A prime enzyme
LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh...  Read full blog post.

Understanding DNA Recombination with Cre-Lox
Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan...  Read full blog post.

Cre/Lox: The Genomic Utility Knife
Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex...  Read full blog post.

LOX propeptide: A novel peptide cancer therapeutic
Lysyl oxidase, also known as LOX, is a copper-dependent enzyme that cross-links collagen and elastin through the oxidative deamination of peptidyl lysine (collagen and elastin) and hydroxylysine (collagen only) residues, thereby playing a critical rol...  Read full blog post.

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Bioinformatics

Gene Symbol LOX