| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB100-2530AF405 | |||
| Alexa Fluor 488 | NB100-2530AF488 | |||
| Alexa Fluor 647 | NB100-2530AF647 | |||
| Alexa Fluor 700 | NB100-2530AF700 | |||
| Biotin | NB100-2530B | |||
| DyLight 488 | NB100-2530G | |||
| DyLight 550 | NB100-2530R | |||
| DyLight 650 | NB100-2530C | |||
| HRP | NB100-2530H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2530SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-41568SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30327SS | |||
![Western Blot: LOX Antibody [NB100-2527] - LOX secretion and activity in response to tumor cell irradiation. Western blot of LOX zymogen (50 kDa) and active form (32 kDa) in conditioned media derived from irradiated A549 lung carcinoma cells 16-20 h after irradiation and quantification of band intensities from 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-Antibody-Western-Blot-NB100-2527-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: LOX Antibody [NB100-2527] - LOX secretion and activity in response to tumor cell irradiation. Western blot of LOX zymogen (50 kDa) and active form (32 kDa) in conditioned media derived from irradiated A549 lung carcinoma cells 16-20 h after irradiation and quantification of band intensities from 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527] - Expression of Lox and Loxl1-Loxl4 mRNAs in orthotopic rat prostate tumours and in the surrounding normal rat prostate tissue. Representative sections from a 10-day orthotopic AT-1 rat prostate tumour (T), tumour-adjacent normal rat prostate tissue (N), and tumour-free control rat prostate tissue stained for LOX (inserts show LOX staining in higher magnifications). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep19608), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunohistochemistry-Paraffin-NB100-2527-img0017.jpg)
![Simple Western: LOX Antibody [NB100-2527] - Simple Western shows a specific band for LOX in 1.0 mg/ml of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LOX-Antibody-Simple-Western-NB100-2527-img0009.jpg)
![Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527] - LOX in fibrotic mice lung tissue. Primary antibody at 1:100, incubated overnight at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunohistochemistry-Paraffin-NB100-2527-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: LOX Antibody [NB100-2527] - IR-induced LOX promotes the invasive capacity of naive tumor cells. Western blot of LOX in conditioned media derived from untreated and irradiated A549 cells, transfected with siLuc-control or siLOX-RNA. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-Antibody-Knockdown-Validated-NB100-2527-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2527] - LOX antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/LOX-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2527-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: LOX Antibody [NB100-2527] - IR-enhanced LOX in tumor xenografts. Tumor tissue from A549-derived tumor xenografts treated with 1 x 10 Gy and 2 x 10 Gy (with 12 h between fractions) and evaluated by immunohistochemistry for LOX at different time points after irradiation. Whole tumor sections were quantified for specific LOX-staining intensity. Each treatment group consists of 3 animals. At least 3 sections per tumor/animal were analyzed. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunohistochemistry-NB100-2527-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2527] - Staining in human fibroblasts. Image from verified customer review.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2527-img0013.jpg)
![Western Blot: LOX Antibody [NB100-2527] - Analysis of LOX in human kidney using NB100-2527.](http://images.novusbio.com/fullsize/LOX-Antibody-Western-Blot-NB100-2527-img0005.jpg)
![Immunohistochemistry: LOX Antibody [NB100-2527] - Staining of LOX in mouse stomach.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunohistochemistry-NB100-2527-img0006.jpg)
![Western Blot: LOX Antibody [NB100-2527] - Patupilone counteracts hypoxia- but not IR-induced LOX-secretion. (A) Western blot of basal & IR-induced LOX in conditioned media derived from patupilone-untreated or patupilone-pretreated (0.5 nM) cells & quantification of band intensities from 3 independent experiments. (B) Western blot of basal & hypoxia-induced LOX in conditioned media derived from patupilone-untreated or patupilone-pretreated (0.5 nM) cells & quantification of band intensities from 3 independent experiments. (C) LOX gene transcription 16 h after irradiation & hypoxia, determined by RT-PCR, averaged over 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2527_rabbit-polyclonal-lox-antibody-310202415363721.jpg)
![Western Blot: LOX Antibody [NB100-2530] - Detection of LOX in mouse kidney lysate using NB 100-2530 (1:1,000). ECL detection was 15 sec.](http://images.novusbio.com/fullsize/LOX-Antibody-Western-Blot-NB100-2530-img0006.jpg "Western Blot: LOX Antibody [NB100-2530] - Detection of LOX in mouse kidney lysate using NB 100-2530 (1:1,000). ECL detection was 15 sec.")
![Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2530] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LOX [NB100-2530] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2530-img0009.jpg)
![Immunohistochemistry-Paraffin: LOX Antibody [NB100-2530] - Lysyl Oxidase (LOX) expression in malignant and non-malignant human prostate tissue. Consecutive sections from non-malignant prostate tissue stained with in situ hybridization or immunohistochemistry for LOX. Note that mRNA (brown dots) and protein (brown) expression in individual glands were related, in the stroma in contrast mRNA was generally low but protein often detected (original magnifications x200). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140985), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunohistochemistry-Paraffin-NB100-2530-img0010.jpg)
![Immunohistochemistry: LOX Antibody [NB100-2530] - Human placental villi, 40X. Staining of placental trophoblasts using NB100-2530 at 10 ug/ml.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunohistochemistry-NB100-2530-img0007.jpg)
![Simple Western: LOX Antibody [NB100-2530] - Simple Western lane view shows a specific band for Lox in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LOX-Antibody-Simple-Western-NB100-2530-img0008.jpg)
![Immunohistochemistry: LOX Antibody - BSA Free [NB100-2530] - alpha -SMA, LOX, & COL1A1 immunostaining in human thyroid cancers. Representative thyroid tumors serial sections from two different patients stained by IHC for alpha -SMA, COL1A1, & LOX protein expression & localization. In the top panel, the tumor edge/invasive front is specifically shown (scale bar 500 µm), while lower panel has higher magnification (scale bar 200 µm). * clusters of tumor invading cells detaching from the principal tumor mass (T). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2530_rabbit-polyclonal-lox-antibody-310202415293322.jpg)
![Immunohistochemistry: LOX Antibody - BSA Free [NB100-2530] - Expression of LOX in primary nasopharyngeal carcinoma (IHC staining) Strong expression(a) magnification, ×200; (A) magnification, ×400; Moderate expression: (b) magnification, ×200; (B) magnification, ×400; Weak expression: (c) magnification, ×200; (C) magnification, ×400; Negative: (d) magnification, ×200; (D) magnification, ×400. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.6996), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2530_rabbit-polyclonal-lox-antibody-31020241539749.jpg)
![Immunohistochemistry: LOX Antibody - BSA Free [NB100-2530] - alpha -SMA, LOX, & COL1A1 immunostaining in human thyroid cancers. Representative thyroid tumors serial sections from two different patients stained by IHC for alpha -SMA, COL1A1, & LOX protein expression & localization. In the top panel, the tumor edge/invasive front is specifically shown (scale bar 500 µm), while lower panel has higher magnification (scale bar 200 µm). * clusters of tumor invading cells detaching from the principal tumor mass (T). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2530_rabbit-polyclonal-lox-antibody-310202415395919.jpg)
![Immunohistochemistry-Paraffin: LOX Antibody - BSA Free [NB100-2530] - Elastin & elastin-related proteins. Tile scans of sheep aortic valve sections stained with Russell Movat's pentachrome (mature elastic fibers in black) & antibodies against proteins involved in elastic fiber formation, including the core protein (tropo)elastin, fibronectin, the microfibrillar proteins fibrillin-1 & fibrillin-2, & cross-linking proteins fibulin-4, fibulin-5, lysyl oxidase (LOX), & Elastin microfibril interfacer 1 (EMILIN-1). Scale bars, 1 mm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30159315), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2530_rabbit-polyclonal-lox-antibody-310202415395921.jpg)
![Immunohistochemistry-Paraffin: LOX Antibody - BSA Free [NB100-2530] - Lysyl Oxidase (LOX) expression in malignant & non-malignant human prostate tissue.(A) Representative immunohistochemical staining of LOX (brown) in sections of non-malignant prostate tissue specimens (TINT epithelium & TINT stroma) from two prostate cancer patients (original magnifications x 200). Case A show weak epithelial staining (score 1) & Case B strong epithelial staining (score 3). (B) Consecutive sections from non-malignant prostate tissue stained with in situ hybridization or immunohistochemistry for LOX. Note that mRNA (brown dots) & protein (brown) expression in individual glands were related, in the stroma in contrast mRNA was generally low but protein often detected (original magnifications x200). (C) LOX mRNA expression in non-malignant prostate tissue (TINT) (n = 12), in primary prostate tumor tissue (n = 12), & in castration-resistant bone metastases (CRPC) (n = 30) using Illumina gene expression array. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26501565), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2530_rabbit-polyclonal-lox-antibody-310202415532099.jpg)
![Immunohistochemistry-Paraffin: LOX Antibody - BSA Free [NB100-2530] - Lysyl Oxidase (LOX) expression in malignant & non-malignant human prostate tissue.(A) Representative immunohistochemical staining of LOX (brown) in sections of non-malignant prostate tissue specimens (TINT epithelium & TINT stroma) from two prostate cancer patients (original magnifications x 200). Case A show weak epithelial staining (score 1) & Case B strong epithelial staining (score 3). (B) Consecutive sections from non-malignant prostate tissue stained with in situ hybridization or immunohistochemistry for LOX. Note that mRNA (brown dots) & protein (brown) expression in individual glands were related, in the stroma in contrast mRNA was generally low but protein often detected (original magnifications x200). (C) LOX mRNA expression in non-malignant prostate tissue (TINT) (n = 12), in primary prostate tumor tissue (n = 12), & in castration-resistant bone metastases (CRPC) (n = 30) using Illumina gene expression array. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26501565), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2530_rabbit-polyclonal-lox-antibody-310202415533822.jpg)
![Western Blot: LOX Antibody [NBP2-24877] - Analysis of mouse ear lysate when LOX antibody (NBP2-24877) was used at 1:1000. The band shown is the expected inactive 50 kD proenzyme.](http://images.novusbio.com/fullsize/LOX-Antibody-Western-Blot-NBP2-24877-img0006.jpg "Western Blot: LOX Antibody [NBP2-24877] - Analysis of mouse ear lysate when LOX antibody (NBP2-24877) was used at 1:1000. The band shown is the expected inactive 50 kD proenzyme.")
![Immunocytochemistry/Immunofluorescence: LOX Antibody [NBP2-24877] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LOX (NBP2-24877) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24877-img0007.jpg)
![Immunohistochemistry-Paraffin: LOX Antibody [NBP2-24877] - Analysis of LOX in formalin-fixed, paraffin-embedded mouse skin using an isotype control (top) and NBP2-24877 (bottom) at 1:100.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunohistochemistry-Paraffin-NBP2-24877-img0002.jpg)
![Western Blot: LOX Antibody [NBP2-24877] - Analysis of LOX in A) human skin and B) mouse ear skin lysate using NBP2-24877 at 1:500. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/LOX-Antibody-Western-Blot-NBP2-24877-img0001.jpg)
![Western Blot: LOX propeptide Antibody [NB110-41568] - Detection of LOPP in MC3T3-E1 cell lysate.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NB110-41568-img0012.jpg "Western Blot: LOX propeptide Antibody [NB110-41568] - Detection of LOPP in MC3T3-E1 cell lysate.")
![Immunohistochemistry: LOX propeptide Antibody [NB110-41568] - LOX-PP was over-expressed in the vascular wall and in VSMC from TgLOX mice. (a) Representative immunohistochemical analysis showing LOX-PP staining (brown color) in aorta from both wild-type and TgLOX mice. The indicated areas are shown at high magnification (bars = 20 um). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-31312-w) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunohistochemistry-NB110-41568-img0017.jpg)
![Western Blot: LOX propeptide Antibody - BSA Free [NB110-41568] - LOX propeptide Antibody [NB110-41568] - Human VSMC were transduced with a lentiviral vector encoding for full-length LOX (pLOX; black bars), LOX-PP (pLOX-PP; grey bars) or with the corresponding empty vector (pLVX; V; white bars). (a) Immunoblots corresponding to mature LOX (mLOX; left panel) & LOX-PP (right panel). The position of the pro-enzyme (ProLOX, right panel), detected with the antibody against the propeptide. GAPDH was analyzed as a loading control. Displayed blots are not cropped from different gels or different parts of the same gel. Representative immunoblots from 3 independent experiments were shown. Image collected & cropped by Cite Ab from the following publication (//pubmed.ncbi.nlm.nih.gov/30185869/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NB110-41568-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: LOX propeptide Antibody [NB110-41568] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-LOX propeptide at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunocytochemistry-Immunofluorescence-NB110-41568-img0015.jpg)
![Western Blot: LOX propeptide Antibody [NB110-41568] - Total protein from human and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-LOX propeptide in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NB110-41568-img0014.jpg)
![Immunohistochemistry-Paraffin: LOX propeptide Antibody [NB110-41568] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti- LOX propeptide (LOX-PP) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This LOX-PP antibody generated a strong cytoplasmic staining in the epithelial and other cells with a majority of the signal localizing to luminal end of columner epithelial cells. There appears to be some nuclear positivity also which might be a nuclear variant of LOX-PP.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunohistochemistry-Paraffin-NB110-41568-img0011.jpg)
![Immunohistochemistry: LOX propeptide Antibody - BSA Free [NB110-41568] - Mature LOX & LOX-PP were over-expressed in the vascular wall & in VSMC from TgLOX mice. (a) Representative immunohistochemical analysis showing LOX & LOX-PP staining (brown color) in aorta from both wild-type & TgLOX mice. The indicated areas are shown at high magnification (bars = 20 µm). (b,c) Mature LOX & LOX-PP protein levels were determined by Western-blot in VSMC supernatants from these animals. The position of the pro-enzyme (ProLOX), mature LOX (mLOX) & LOX-PP forms are indicated. GAPDH was analyzed as loading control. Representative immunoblots from 3 independent experiments were shown. WT: wild-type; Tg: TgLOX. Displayed blots are not cropped from different gels or different parts of the same gel & images conform the digital image & integrity policies of the journal. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30185869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41568_rabbit-polyclonal-lox-propeptide-antibody-3102024162492.jpg)
![Western Blot: LOX propeptide Antibody - BSA Free [NB110-41568] - Over-expression of LOX-PP did not affect VSMC proliferation. Human VSMC were transduced with a lentiviral vector encoding for full-length LOX (pLOX; black bars), LOX-PP (pLOX-PP; grey bars) or with the corresponding empty vector (pLVX; V; white bars). (a) Immunoblots corresponding to mature LOX (mLOX; left panel) & LOX-PP (right panel) are shown. The position of the pro-enzyme (ProLOX, right panel), detected with the antibody against the propeptide, was also indicated. GAPDH was analyzed as a loading control. Displayed blots are not cropped from different gels or different parts of the same gel & images conform the digital image & integrity policies of the journal. Representative immunoblots from 3 independent experiments were shown. (b) Transduced VSMC were serum-starved for 24 h & then stimulated with 20% FCS. Cell proliferation was evaluated by the [3H]-thymidine incorporation method (left panel) or by cell count (right panel) in these cells. Results represented as mean ± SD. *P < 0.003 vs. pLVX (Kruskal-Wallis; at least n = 6). (c) [3H]-thymidine incorporation into DNA assessed in human VSMC transduced with lentiviral particles for full-length LOX (pLOX; black bars), or the corresponding empty vector (pLVX; white bars) treated or not with BAPN (500 µM). Results are represented as mean ± SD. P < 0.0001: * vs. pLVX; #vs. pLOX transduced cells (Two-way ANOVA; at least n = 9). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30185869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41568_rabbit-polyclonal-lox-propeptide-antibody-31020241624915.jpg)
![Western Blot: LOX propeptide Antibody [NBP1-30327] - LOX-PP reduces CIN85 mono-ubiquitination and ability to interact with c-Cbl. HEK293T cells were transfected with AMAP1-FLAG, CIN85, HA-c-Cbl, HA-ubiquitin and LOX-PP-GST (PP-GST) as indicated and subjected to a ubiquitination assay. FLAG-tagged AMAP1 was immunoprecipitated and total whole cell extracts were subjected to WB with the indicated antibodies (lower panel). Data were quantified and relative mono-ubiquitination of AMAP1 with and without LOX-PP was determined by averaging the results of three independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0077288), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NBP1-30327-img0010.jpg "Western Blot: LOX propeptide Antibody [NBP1-30327] - LOX-PP reduces CIN85 mono-ubiquitination and ability to interact with c-Cbl. HEK293T cells were transfected with AMAP1-FLAG, CIN85, HA-c-Cbl, HA-ubiquitin and LOX-PP-GST (PP-GST) as indicated and subjected to a ubiquitination assay. FLAG-tagged AMAP1 was immunoprecipitated and total whole cell extracts were subjected to WB with the indicated antibodies (lower panel). Data were quantified and relative mono-ubiquitination of AMAP1 with and without LOX-PP was determined by averaging the results of three independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0077288), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: LOX propeptide Antibody [NBP1-30327] - Staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30327-img0003.jpg)
![Flow Cytometry: LOX propeptide Antibody [NBP1-30327] - An intracellular stain was performed on HeLa with LOX propeptide Antibody NBP1-30327 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Flow-Cytometry-NBP1-30327-img0009.jpg)
![Western Blot: LOX propeptide Antibody [NBP1-30327] - Analysis of LOX propeptide on MDA-MB-231 using NBP1-30327.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NBP1-30327-img0006.jpg)
![Simple Western: LOX propeptide Antibody [NBP1-30327] - Simple Western lane view shows a specific band for LOX in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Simple-Western-NBP1-30327-img0008.jpg)
![Western Blot: LOX Antibody (9K10Z3) [NBP3-15396] - Western blot analysis of extracts of various cell lines, using LOX Rabbit mAb (NBP3-15396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/LOX-Antibody-9K10Z3-Western-Blot-NBP3-15396-img0003.jpg "Western Blot: LOX Antibody (9K10Z3) [NBP3-15396] - Western blot analysis of extracts of various cell lines, using LOX Rabbit mAb (NBP3-15396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: LOX Antibody (9K10Z3) [NBP3-15396] - Immunohistochemistry of paraffin-embedded human colon using LOX Rabbit mAb (NBP3-15396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LOX-Antibody-9K10Z3-Immunohistochemistry-Paraffin-NBP3-15396-img0002.jpg)
![Immunohistochemistry-Paraffin: LOX Antibody (9K10Z3) [NBP3-15396] - Immunohistochemistry of paraffin-embedded rat kidney using LOX Rabbit mAb (NBP3-15396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LOX-Antibody-9K10Z3-Immunohistochemistry-Paraffin-NBP3-15396-img0001.jpg)
![Immunoprecipitation: LOX Antibody (9K10Z3) [NBP3-15396] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug LOX antibody (NBP3-15396). Western blot was performed from the immunoprecipitate using LOX antibody (NBP3-15396) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/LOX-Antibody-9K10Z3-Immunoprecipitation-NBP3-15396-img0004.jpg)
![SDS-Page: Recombinant Human LOX Protein [NBP2-59887] - 15% SDS-PAGE](http://images.novusbio.com/fullsize/Recombinant-Human-LOX-Protein-SDS-Page-NBP2-59887-img0001.jpg "SDS-Page: Recombinant Human LOX Protein [NBP2-59887] - 15% SDS-PAGE")
![Immunocytochemistry/Immunofluorescence: LOX Antibody [NBP2-55446] - Staining of human cell line BJ shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/LOX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55446-img0001.jpg "Immunocytochemistry/Immunofluorescence: LOX Antibody [NBP2-55446] - Staining of human cell line BJ shows localization to endoplasmic reticulum.")