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LMO2 Antibody (OTI1G10)

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Western Blot: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMO2.
Western Blot: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMO2.
Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Human prostate tissue (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Human bladder. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: LMO2 Antibody (1G10) [NBP2-45787] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71157AF350
Alexa Fluor 405
NBP2-71157AF405
Alexa Fluor 488
NBP2-71157AF488
Alexa Fluor 532
NBP2-71157AF532
Alexa Fluor 594
NBP2-71157AF594
Alexa Fluor 647
NBP2-71157AF647
Alexa Fluor 700
NBP2-71157AF700
Alexa Fluor 750
NBP2-71157AF750
Allophycocyanin
NBP2-71157APC
Biotin
NBP2-71157B
CoraFluor 1
NBP2-71157CL1
DyLight 350
NBP2-71157UV
DyLight 405
NBP2-71157V
DyLight 488
NBP2-71157G
DyLight 550
NBP2-71157R
DyLight 594
NBP2-71157DL594
DyLight 650
NBP2-71157C
DyLight 680
NBP2-71157FR
DyLight 755
NBP2-71157IR
FITC
NBP2-71157F
HRP
NBP2-71157H
Janelia Fluor 525
NBP2-71157JF525
Janelia Fluor 549
NBP2-71157JF549
Janelia Fluor 585
NBP2-71157JF585
Janelia Fluor 635
NBP2-71157JF635
Janelia Fluor 646
NBP2-71157JF646
Janelia Fluor 669
NBP2-71157JF669
PE
NBP2-71157PE
PerCP
NBP2-71157PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71157
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LMO2 Antibody (OTI1G10) Summary

Immunogen
Full length human recombinant protein of human LMO2(NP_005565) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LMO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:4000
Theoretical MW
18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LMO2 Antibody (OTI1G10)

  • Cysteine-rich protein TTG-2
  • LIM domain only 2 (rhombotin-like 1)
  • LIM domain only protein 2
  • LMO2
  • LMO-2
  • RBTN2
  • RBTN2rhombotin-2
  • RBTNL1
  • RBTNL1rhombotin-like 1
  • RHOM2
  • RHOM2T-cell translocation protein 2
  • T-cell translocation gene 2
  • TTG2
  • TTG2rhombotin 2

Background

LMO2 is a highly conserved oncoprotein that is expressed in human normal germinal-center B cells and in B-cell lymphomas. LMO2 also has a crucial role in hematopoietic development by being necessary for yolk sac erythropoiesis. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number of T-cell acute lymphoblastic leukemia-specific translocations occur.

LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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LMO2 Antibody
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NBP2-45787
LMO2 Antibody (OTI1G10)
Species: Hu, Mu, Rt
Applications: WB, IHC

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Bioinformatics

Gene Symbol LMO2