![Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 induces demethylation and re-expression of tumor suppressor genes. Cells were treated with SH-I-14 for 48 hr and subjected to western blot analysis. The lysates from the cells treated with SH-I-14 for 48 hr were analyzed by western blot with indicated antibodies. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/4054), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-NBP1-80833-img0015.jpg "Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 induces demethylation and re-expression of tumor suppressor genes. Cells were treated with SH-I-14 for 48 hr and subjected to western blot analysis. The lysates from the cells treated with SH-I-14 for 48 hr were analyzed by western blot with indicated antibodies. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/4054), licensed under a CC-BY license.")
| Reactivity | Hu, Rt, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: HSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPE |
| Predicted Species | Mouse (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PDLIM4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB reported in scientific literature (PMID: 29137356). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP1-80833] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80833-img0008.jpg "Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP1-80833] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunohistochemistry-Paraffin-NBP1-80833-img0014.jpg "Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.")
![Western Blot: PDLIM4 Antibody [NBP1-80833] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-NBP1-80833-img0003.jpg "Western Blot: PDLIM4 Antibody [NBP1-80833] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Western Blot: PDLIM4 Antibody [NBP1-80833] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Western-Blot-NBP1-80833-img0011.jpg "Western Blot: PDLIM4 Antibody [NBP1-80833] - Analysis in human cell line RH-30.")
![Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human Fallopian tube shows weak to moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunohistochemistry-Paraffin-NBP1-80833-img0012.jpg "Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human Fallopian tube shows weak to moderate membranous and cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PDLIM4-Antibody-Immunohistochemistry-Paraffin-NBP1-80833-img0013.jpg "Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.")
![Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 reduces the in vivo growth of tumors in xenograft mice bearing tumors of MDA-MB-231 cellsA. SH-I-14 (10 mg/kg) or DMSO was injected into peritoneal cavity of mouse with MDA-MB-231 tumors three times per week from 8-days after tumor cell injection. The control mice were euthanized earlier due to large tumor size. Tumor volumes were measured as described in Materials & Methods. Data are shown as mean±SD. **P < 0.01. B. SH-I-14 reduces acetyl-STAT3 & induces the expression of VHL & PDLIM4 protein in vivo. The MDA-MB-231 xenograft mice (3 mice per group) were administered with 10 mg/kg of SH-I-14 daily for 5 days & lysates from each tumor were subjected to western blot analysis as indicated. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29137356), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-80833_rabbit-polyclonal-pdlim4-antibody-31020241539254.jpg "Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 reduces the in vivo growth of tumors in xenograft mice bearing tumors of MDA-MB-231 cellsA. SH-I-14 (10 mg/kg) or DMSO was injected into peritoneal cavity of mouse with MDA-MB-231 tumors three times per week from 8-days after tumor cell injection. The control mice were euthanized earlier due to large tumor size. Tumor volumes were measured as described in Materials & Methods. Data are shown as mean±SD. **P < 0.01. B. SH-I-14 reduces acetyl-STAT3 & induces the expression of VHL & PDLIM4 protein in vivo. The MDA-MB-231 xenograft mice (3 mice per group) were administered with 10 mg/kg of SH-I-14 daily for 5 days & lysates from each tumor were subjected to western blot analysis as indicated. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29137356), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry LMO2 Antibody [Unconjugated]](https://images.novusbio.com/images2/LMO2_AF2726_Immunocytochemistry_10616.jpg)
![Flow Cytometry LMO2 Antibody [Unconjugated]](https://images.novusbio.com/images2/LMO2_AF2726_Flow_Cytometry_22133.jpg)
![Flow Cytometry LMO2 Antibody [Unconjugated]](https://images.novusbio.com/images2/LMO2_AF2726_Flow_Cytometry_22134.jpg)
![Immunohistochemistry Complement Factor H Antibody [Unconjugated]](https://images.novusbio.com/images2/Complement_Factor_H_AF4779_Immunohistochemistry_7182.jpg)
![N/A SLPI [HRP]](https://images.novusbio.com/images2/DATA_SLPI_DPI00_ELISA_792.jpg)
![N/A SLPI [HRP]](https://images.novusbio.com/images2/SLPI_DPI00_ELISA_160.jpg)
![N/A CCL27/CTACK [HRP]](https://images.novusbio.com/images2/CCL27_DCC270_ELISA_54.jpg)
![N/A CCL27/CTACK [HRP]](https://images.novusbio.com/images2/DATA_CCL27_DCC270_ELISA_615.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")