LIX Antibody (61905) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-9 kDa member of the Intercrine alpha (or CxC) family of chemokines.  It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium.  As a chemokine, LIX demonstrates chemokinetic properties.  It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages.  Notably, circulating LIX is not derived from fibroblasts, but platelets.  This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX.  Mouse LIX is synthesized as a 132 amino acid (aa) precursor that contains a 40 aa signal sequence, a 78 aa mature region (aa 41-118), and a cleavable 14 aa C-terminus.  The mature region possesses an ELR/GluLeuArg motif between aa 50-52, and an alpha -family characteristic CxC motif between aa 53-55.  Although there are no known splice variants of mouse LIX, considerable proteolytic processing occurs at both the N- and C-termini over aa 41-132.  This may reduce the MW in SDS-PAGE by as much as 3 kDa.  The majority of LIX appears to start between aa 47-50, and this is positively correlated with bioactivity.  Over aa 41-118, mouse LIX shares 73% aa sequence identity with rat LIX.  Although not a strict ortholog, mouse LIX shares 63% aa sequence identity with human GCP-2.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 9 applications: ELISA Capture, ELISA Development, ELISA Development (Capture), IHC, IHC-Fr, IHC-P, In vivo assay, Neutralization, Western Blot.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using MAB433	Applications	Species
Zhuang, W;Zhou, J;Zhong, L;Lv, J;Zhong, X;Liu, G;Xie, L;Wang, C;Saimaier, K;Han, S;Shi, C;Hua, Q;Zhang, R;Xie, X;Du, C; CXCR1 drives the pathogenesis of EAE and ARDS via boosting dendritic cells-dependent inflammation Cell death & disease 2023-09-14 [PMID: 37709757] (In vivo assay, Mouse)	In vivo assay	Mouse
Chen, C;Lin, LY;Chen, JW;Chang, TT; CXCL5 suppression recovers neovascularization and accelerates wound healing in diabetes mellitus Cardiovascular diabetology 2023-07-07 [PMID: 37420254] (Neutralization, Mouse)	Neutralization	Mouse
G Xiao, R Kumar, Y Komuro, J Burguet, V Kakarla, I Azizkhania, SA Sheth, CK Williams, XR Zhang, M Macknicki, A Brumm, R Kawaguchi, P Mai, N Kaneko, HV Vinters, ST Carmichael, LA Havton, C DeCarli, JD Hinman IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury Cell Reports, 2022-12-20;41(12):111848. 2022-12-20 [PMID: 36543124] (IHC, Mouse)	IHC	Mouse
Z Pang, R Raudonis, C McCormick, Z Cheng Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection Infect. Immun., 2019-12-17;0(0):. 2019-12-17 [PMID: 31611276] (ELISA Capture, Mouse)	ELISA Capture	Mouse
R Romero-Mor, KJ Curtis, TR Coughlin, M Cristina M, S Dutta, A Natarajan, BA Facchine, KM Jackson, L Nystrom, J Li, W Kaliney, GL Niebur, LE Littlepage The CXCL5/CXCR2 axis is sufficient to promote breast cancer colonization during bone metastasis Nat Commun, 2019-09-27;10(1):4404. 2019-09-27 [PMID: 31562303] (IHC-P, Mouse)	IHC-P	Mouse
Tamara Girbl, Tchern Lenn, Lorena Perez, Loïc Rolas, Anna Barkaway, Aude Thiriot et al. Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis Immunity 12/18/2018 [PMID: 30446388]
Hongpeng Jia Pulmonary Epithelial TLR4 Activation Leads to Lung Injury in Neonatal Necrotizing Enterocolitis J Immunol, 2016-06-15;0(0):. 2016-06-15 [PMID: 27307558] (Neutralization, Mouse)	Neutralization	Mouse
Junkins R, MacNeil A, Wu Z, McCormick C, Lin T Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections. J Immunol, 2013-04-15;190(10):5178-86. 2013-04-15 [PMID: 23589609] (ELISA Development (Capture), Mouse)	ELISA Development (Capture)	Mouse
Song J, Wu C, Zhang X, Sorokin L In vivo processing of CXCL5 (LIX) by matrix metalloproteinase (MMP)-2 and MMP-9 promotes early neutrophil recruitment in IL-1beta-induced peritonitis. J Immunol, 2012-12-07;190(1):401-10. 2012-12-07 [PMID: 23225890] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S, Thompson TC Castration-induced changes in mouse epididymal white adipose tissue. Mol. Cell. Endocrinol., 2011-07-12;345(1):58-67. 2011-07-12 [PMID: 21782885] (IHC-P, Mouse)	IHC-P	Mouse
Halpern Jennifer L, Kilbarger Amy, Lynch Conor C. Mesenchymal stem cells promote mammary cancer cell migRation in vitro via the CXCR2 receptor. Cancer Letters 1/1/2011 [PMID: 21601983]
Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation. J. Immunol., 2005-08-15;175(4):2589-97. 2005-08-15 [PMID: 16081833] (Western Blot, Mouse)	Western Blot	Mouse
Maheshwari A, Christensen RD, Calhoun DA Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model. Fetal Pediatr Pathol, 2004-03-01;23(2):145-57. 2004-03-01 [PMID: 15768860] (ELISA Development, Mouse)	ELISA Development	Mouse
Jeyaseelan S, Chu HW, Young SK, Worthen GS Transcriptional profiling of lipopolysaccharide-induced acute lung injury. Infect. Immun., 2004-12-01;72(12):7247-56. 2004-12-01 [PMID: 15557650] (ELISA Development, Neutralization, Mouse)	ELISA Development, Neutralization	Mouse
Show All 14 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Mouse	09/30/2021	View Summary Application Western Blot Sample Tested fibroblasts Species Mouse Lot 61905

Array MAB433

• LIX Antibodies
• LIX ELISAs
• LIX Proteins and Enzymes