Name: LAMP-1/CD107a Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF4320
Rating: 5 (2 reviews)

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Date

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reviewed by:
Verified Customer

Mouse

11/21/2019

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Summary

Application	Western Blot
Sample Tested	Primary oligodendrocyte cultures,Primary sorted Oligodendrocytes
Species	Mouse
Lot	ZPS0218121

Comments

Dilution of 0.2 mg/mL stock: 1:1000

Great antibody for each application tested so far on mouse tissue and cells: wb, IHC-Fr, ICC

Immunohistochemistry-Frozen LAMP-1/CD107a AF4320

IHC-Fr

Application	Immunohistochemistry-Frozen
Sample Tested	Adult spinal cord,Primary culture of oligodendrocytes
Species	Mouse
Lot	ZPS0218121

Dilution of 0.2 mg/mL stock: 1:40

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	WB, CyTOF-ready, Flow, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free AF4320-SP

Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse LAMP1/CD107a Leu25-Asn370 Accession # P11438
Specificity	Detects mouse LAMP1/CD107a Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant human LAMP1 is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	LAMP1
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

LAMP-1/CD107a Antibody [Unconjugated]

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LAMP-1/CD107a Antibody [Unconjugated] Summary

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Publications for LAMP-1/CD107a Antibody (AF4320)(16)

Reviews for LAMP-1/CD107a Antibody (AF4320) (2) 52

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Bioinformatics

Publications using AF4320	Applications	Species
Jing Li, Qi Huang, Minling Lv, Wenfeng Ma, Jialing Sun, Xin Zhong et al. Role of liensinine in sensitivity of activated macrophages to ferroptosis and in acute liver injury Cell Death Discovery 6/23/2023 [PMID: 37353487]
Salas-Lucia F, Fekete C, Sinkó R et al. Axonal T3 uptake and transport can trigger thyroid hormone signaling in the brain eLife 2023-05-19 [PMID: 37204837]
J Watson, HR Ferguson, RM Brady, J Ferguson, P Fullwood, H Mo, KH Bexley, D Knight, G Howell, JM Schwartz, MP Smith, C Francavill Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival Nature Communications, 2022-11-03;13(1):6589. 2022-11-03 [PMID: 36329028] (ICC, Human)	ICC	Human
J DeGeer, AL Datwyler, C Rickenbach, A Ommer, D Gerber, C Fimiani, J Gerber, JA Pereira, U Suter Ral GTPases are critical regulators of spinal cord myelination and homeostasis Cell Reports, 2022-09-27;40(13):111413. 2022-09-27 [PMID: 36170840] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
T Wan, W Zhu, Y Zhao, X Zhang, R Ye, M Zuo, P Xu, Z Huang, C Zhang, Y Xie, X Liu Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice Nature Communications, 2022-03-03;13(1):1134. 2022-03-03 [PMID: 35241660] (ICC, Mouse)	ICC	Mouse
Annangi B, Lu Z, Bruniaux J et al. Macrophage autophagy protects mice from cerium oxide nanoparticle-induced lung fibrosis Particle and Fibre Toxicology 2021-12-01 [PMID: 33526046]
W Feng, Y Zhang, Z Wang, H Xu, T Wu, C Marshall, J Gao, M Xiao Microglia prevent beta-amyloid plaque formation in the early stage of an Alzheimer's disease mouse model with suppression of glymphatic clearance Alzheimers Res Ther, 2020-10-02;12(1):125. 2020-10-02 [PMID: 33008458] (IHC, Mouse)	IHC	Mouse
S Pal-Ghosh, G Tadvalkar, BA Karpinski, MA Stepp Diurnal Control of Sensory Axon Growth and Shedding in the Mouse Cornea Invest. Ophthalmol. Vis. Sci., 2020-09-01;61(11):1. 2020-09-01 [PMID: 32870244] (IHC, Mouse)	IHC	Mouse
Kuang L, Hashimoto K, Huang EJ et al. Frontotemporal dementia nonsense mutation of progranulin rescued by aminoglycosides Hum. Mol. Genet. 1/8/2020 [PMID: 31913476]
Olivia Majer, Bo Liu, Brian J. Woo, Lieselotte S. M. Kreuk, Erik Van Dis, Gregory M. Barton Release from UNC93B1 reinforces the compartmentalized activation of select TLRs Nature 2019-11-14 [PMID: 31546247]
Olivia Majer, Bo Liu, Lieselotte S. M. Kreuk, Nevan Krogan, Gregory M. Barton UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity Nature 2019-11-14 [PMID: 31546246]
Mathieu Daynac, Malek Chouchane, Hannah Y. Collins, Nicole E. Murphy, Noemi Andor, Jianqin Niu et al. Lgl1 controls NG2 endocytic pathway to regulate oligodendrocyte differentiation and asymmetric cell division and gliomagenesis Nature Communications 2018-08-21 [PMID: 30131568]
Pal-Ghosh S, Tadvalkar G, Stepp MA. Alterations in Corneal Sensory Nerves During Homeostasis, Aging, and After Injury in Mice Lacking the Heparan Sulfate Proteoglycan Syndecan-1. Invest. Ophthalmol. Vis. Sci. 10/1/2017 [PMID: 28973369]
M Koike, M Shibata, T Sunabori, J Yamaguchi, K Sakimura, M Komatsu, K Tanaka, Y Uchiyama Purkinje Cells Are More Vulnerable to the Specific Depletion of Cathepsin D Than to That of Atg7 Am. J. Pathol., 2017-05-11;0(0):. 2017-05-11 [PMID: 28502476] (IHC-P, Mouse)	IHC-P	Mouse
J Zhang, JL Johnson, J He, G Napolitano, M Ramadass, C Rocca, WB Kiosses, C Bucci, Q Xin, E Gavathioti, AM Cuervo, S Cherqui, SD Catz Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A J. Biol. Chem., 2017-05-02;292(25):10328-10346. 2017-05-02 [PMID: 28465352] (ICC, Mouse)	ICC	Mouse
Cyrill Bussy, Erwan Paineau, Julien Cambedouzou, Nathalie Brun, Claudie Mory, Barbara Fayard et al. Intracellular fate of carbon nanotubes inside murine macrophages: pH-dependent detachment of iron catalyst nanoparticles Particle and Fibre Toxicology 2013-12-01 [PMID: 23800198]
Show All 16 Publications. Collapse Publications.

Gene Symbol	LAMP1
Uniprot	P11438()