![Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-212024939180.jpg "Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
| Reactivity | Hu, Ca, FeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1731 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KDM6A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Western Blot Jak1 Antibody (413104) [Unconjugated]](https://images.novusbio.com/images2/Jak1_MAB4260_Western_Blot_6164.jpg)
![Immunohistochemistry Jak1 Antibody (413104) [Unconjugated]](https://images.novusbio.com/images2/Jak1_MAB4260_Immunohistochemistry_7021.jpg)
![Intracellular Staining by Flow Cytometry Jak1 Antibody (413104) [Unconjugated]](https://images.novusbio.com/images2/Jak1_MAB4260_Flow_Cytometry_8448.jpg)
Secondary Antibodies |
Isotype Controls |
|
Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KDM6A |
![Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249342732.jpg "Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249415911.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249362762.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis. Sample: Paraffin-embedded cat tissue. KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249403857.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis. Sample: Paraffin-embedded cat tissue. KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249371583.jpg "Immunocytochemistry/Immunofluorescence: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Non-transfected (-) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249422644.jpg "Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Non-transfected (-) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249393539.jpg "Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot EZH2/KMT6 Antibody [Unconjugated]](https://images.novusbio.com/images2/EZH2_AF4767_Western_Blot_12571.jpg)
![Western Blot EZH2/KMT6 Antibody [Unconjugated]](https://images.novusbio.com/images/af4767_human-mouse-ezh2-affinity-purified-polyclonal-ab-4120241240516.jpg)
![Western Blot EZH2/KMT6 Antibody [Unconjugated]](https://images.novusbio.com/images/af4767_human-mouse-ezh2-affinity-purified-polyclonal-ab-4120241240219.jpg)
![Western Blot Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Western_Blot_5829.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11663.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11664.jpg)
![Knockout Validated Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Knockout_Validated_22540.jpg)
![Immunocytochemistry Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Immunocytochemistry__Immunofluorescence_19067.jpg)
![Western Blot Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Western_Blot_13853.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")