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![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining in human bone marrow and skeletal muscle tissues using NBP1-80628 antibody. Corresponding KDM6A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining in human bone marrow and skeletal muscle tissues using NBP1-80628 antibody. Corresponding KDM6A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: KDM6A Antibody [NBP1-80628] - HMLE cells were transduced with either an empty vector (HMLE-Vector), or genes encoding the EMT-inducing transcription factors Snail (HMLE-Snail) or Twist (HMLE-Twist) [4]. Whole-cell lysates were analyzed by immunoblotting for KDM6A. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29029452/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Western-Blot-NBP1-80628-img0022.jpg)
![Western Blot: KDM6A Antibody [NBP1-80628] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Western-Blot-NBP1-80628-img0006.jpg)
![Western Blot: KDM6A Antibody [NBP1-80628] - MCF-7 and MDA-MB-231 whole cell lysates (40 ug/lane). 10% SDS-PAGE. KDM6A Antibody (NBP1-80628) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Western-Blot-NBP1-80628-img0021.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Red: UTX nuclear colocalization with DAPI (in blue). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0012.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0013.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human cervix shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0016.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0017.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0018.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0019.jpg)
![Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-212024939180.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249342732.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249415911.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249362762.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis. Sample: Paraffin-embedded cat tissue. KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249403857.jpg)
![Immunocytochemistry/Immunofluorescence: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - KDM6A antibody [HL1731] detects KDM6A protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KDM6A stained by KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249371583.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Non-transfected (-) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249422644.jpg)
![Western Blot: KDM6A Antibody (HL1731) - Azide and BSA Free [NBP3-25542] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (NBP3-25542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25542_rabbit-kdm6a-mab-hl1731-azide-and-bsa-free-2120249393539.jpg)
![Knockdown Validated: KDM6A Antibody (HL2068) - Azide and BSA Free [NBP3-25543] - Non-transfected (-) and transfected (+) SK-N-SH whole cell extracts (42 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL2068] (NBP3-25543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25543_rabbit-kdm6a-mab-hl2068-azide-and-bsa-free-212024940899.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: KDM6A Antibody (HL2068) - Azide and BSA Free [NBP3-25543] - Non-transfected (-) and transfected (+) SK-N-SH whole cell extracts (42 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL2068] (NBP3-25543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: KDM6A Antibody (HL2068) - Azide and BSA Free [NBP3-25543] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL2068] (NBP3-25543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25543_rabbit-kdm6a-mab-hl2068-azide-and-bsa-free-2120249351388.jpg)
![Western Blot: KDM6A Antibody (HL2068) - Azide and BSA Free [NBP3-25543] - Various whole cell extracts (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL2068] (NBP3-25543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25543_rabbit-kdm6a-mab-hl2068-azide-and-bsa-free-2120249355522.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: KDM6A Antibody (HL2068) - Azide and BSA Free [NBP3-25543] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL2068] (NBP3-25543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25543_rabbit-kdm6a-mab-hl2068-azide-and-bsa-free-212024936272.jpg)
![Immunocytochemistry/Immunofluorescence: KDM6A Antibody (HL2068) - Azide and BSA Free [NBP3-25543] - KDM6A antibody [HL2068] detects KDM6A protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: KDM6A stained by KDM6A antibody [HL2068] (NBP3-25543) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25543_rabbit-kdm6a-mab-hl2068-azide-and-bsa-free-2120249413390.jpg)
![Immunohistochemistry-Paraffin: KDM6A Antibody (HL2068) - Azide and BSA Free [NBP3-25543] - KDM6A antibody [HL2068] detects KDM6A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. KDM6A stained by KDM6A antibody [HL2068] (NBP3-25543) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25543_rabbit-kdm6a-mab-hl2068-azide-and-bsa-free-2120249415942.jpg)
![Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of KDM6A expression in IMR-32.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Western-Blot-H00007403-M05-img0005.jpg "Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of KDM6A expression in IMR-32.")
![Immunocytochemistry/Immunofluorescence: KDM6A Antibody (4E7) [H00007403-M05] - KDM6A Antibody was used (1:50) for labeling and microscopy analysis of the distribution of KDM6A proteins in MCF-7 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Immunocytochemistry-Immunofluorescence-H00007403-M05-img0008.jpg)
![Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Expression in HepG2.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Western-Blot-H00007403-M05-img0002.jpg)
![Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of KDM6A expression in Raw 264.7.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Western-Blot-H00007403-M05-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of monoclonal antibody to KDM6A on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Immunocytochemistry-Immunofluorescence-H00007403-M05-img0004.jpg)
![ELISA: KDM6A Antibody (4E7.) [H00007403-M05] - KDM6A Antibody (4E7) [H00007403-M05] - Detection limit for recombinant GST tagged KDM6A is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-ELISA-H00007403-M05-img0007.jpg)
![Western Blot: KDM6A Antibody [NBP3-05103] - Analysis of extracts of various cell lines, using KDM6A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/KDM6A-Antibody-Western-Blot-NBP3-05103-img0002.jpg "Western Blot: KDM6A Antibody [NBP3-05103] - Analysis of extracts of various cell lines, using KDM6A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP3-05103] - Paraffin-embedded human breast cancer using KDM6A antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP3-05103-img0001.jpg)
![Western Blot: KDM6A Antibody [NBP2-87672] - WB Suggested Anti-UTX Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysateKDM6A is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells](http://images.novusbio.com/fullsize/KDM6A-Antibody-Western-Blot-NBP2-87672-img0001.jpg "Western Blot: KDM6A Antibody [NBP2-87672] - WB Suggested Anti-UTX Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysateKDM6A is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells")
![Western Blot: KDM6A Antibody [NBP2-87672] - Host: Rabbit. Target: KDM6A. Positive control (+): Human Ovary (OV). Negative control (-): Human liver (LI). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/KDM6A-Antibody-Western-Blot-NBP2-87672-img0002.jpg)
![Western Blot: KDM6A Antibody (1612) [NBP2-43665] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 5 % SDS-PAGE KDM6A antibody [1612] dilution: 1:500.](http://images.novusbio.com/fullsize/KDM6A-Antibody-1612-Western-Blot-NBP2-43665-img0001.jpg "Western Blot: KDM6A Antibody (1612) [NBP2-43665] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 5 % SDS-PAGE KDM6A antibody [1612] dilution: 1:500.")
![Western Blot: KDM6A Antibody [NBP2-87670] - WB Suggested Anti-Utx Antibody. Titration: 1.0 ug/ml. Positive Control: Mouse Intestine](http://images.novusbio.com/fullsize/KDM6A-Antibody-Western-Blot-NBP2-87670-img0001.jpg "Western Blot: KDM6A Antibody [NBP2-87670] - WB Suggested Anti-Utx Antibody. Titration: 1.0 ug/ml. Positive Control: Mouse Intestine")
![Western Blot: KDM6A Antibody [NBP2-87671] - WB Suggested Anti-UTX Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate](http://images.novusbio.com/fullsize/KDM6A-Antibody-Western-Blot-NBP2-87671-img0001.jpg "Western Blot: KDM6A Antibody [NBP2-87671] - WB Suggested Anti-UTX Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate")
![Western Blot: KDM6A Overexpression Lysate (Adult Normal) [NBL1-17682] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KDM6A.](http://images.novusbio.com/fullsize/KDM6A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17682-img0002.jpg "Western Blot: KDM6A Overexpression Lysate (Adult Normal) [NBL1-17682] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KDM6A.")