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KDEL Antibody (2C1)

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Western Blot: KDEL Antibody (2C1) [NBP2-61926] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
2C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

KDEL Antibody (2C1) Summary

Immunogen
KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Specificity
Detects KDEL tagged proteins.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
POGLUT2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:25
  • Western Blot 1:1000
Application Notes
A 1:1000 dilution of this antibody was sufficient for detection of KDEL in 10 ug of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for KDEL Antibody (2C1)

  • Endoplasmic reticulum resident protein 58
  • EP58KDEL1
  • ER protein 58
  • ERp58
  • KDEL (Lys-Asp-Glu-Leu) containing 1
  • KDEL motif-containing 1
  • KDEL motif-containing protein 1
  • MGC5302

Background

he endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POGLUT2