Hu,
Mu,
Rt,
Av,
Ch,
In,
Ma,
Pl,
Pm,
YeSpecies Glossary
Reactivity: Hu, Mu, Rt, Av, Ch, In, Ma, Pl, Pm, Ye
WB, ELISA, Flow, ICC/IF, IHC, IP, MS
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, MS
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Western-Blot-NBP1-97469-img0004.jpg "Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).")
![Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - KDEL in human primary fibroblasts. Antibody at 1:600 in PBST, 0.1% triton + 10% DS. O/N incubation at 4C. Donkey anti-mouse Alexa Fluor 488 at 1:500 in PBST, 0.1% triton + 10% DS 1h RT. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Immunocytochemistry-Immunofluorescence-NBP1-97469-img0006.jpg)
![Immunohistochemistry-Paraffin: KDEL Antibody (10C3) [NBP1-97469] - Analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10 ug/mL.](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Immunohistochemistry-Paraffin-NBP1-97469-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - Analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12.](http://images.novusbio.com/fullsize/KDEL-Antibody-10C3-Immunocytochemistry-Immunofluorescence-NBP1-97469-img0005.jpg)
![Western Blot: KDEL Antibody (2C1) [NBP2-61926] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61926). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.](http://images.novusbio.com/fullsize/KDEL-Antibody-2C1-Western-Blot-NBP2-61926-img0002.jpg "Western Blot: KDEL Antibody (2C1) [NBP2-61926] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61926). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.")
![Immunohistochemistry: KDEL Antibody (2C1) [NBP2-61926] - Immunohistochemistry analysis using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Tissue: kidney. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) KDEL Antibody (D) Composite.](http://images.novusbio.com/fullsize/KDEL-Antibody-2C1-Immunohistochemistry-NBP2-61926-img0003.jpg)
![Western Blot: KDEL Antibody (1F2) [NBP2-61924] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2 (NBP2-61924). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61924). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61924) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.](http://images.novusbio.com/fullsize/KDEL-Antibody-1F2-Western-Blot-NBP2-61924-img0002.jpg "Western Blot: KDEL Antibody (1F2) [NBP2-61924] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2 (NBP2-61924). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61924). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61924) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.")
![Western Blot: KDEL Antibody (2D6) [NBP2-61927] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2D6 (NBP2-61927). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61927). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61927) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.](http://images.novusbio.com/fullsize/KDEL-Antibody-2D6-Western-Blot-NBP2-61927-img0002.jpg "Western Blot: KDEL Antibody (2D6) [NBP2-61927] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2D6 (NBP2-61927). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61927). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61927) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.")
![Immunocytochemistry/Immunofluorescence: KDEL Antibody [NBP2-57105] - Staining of human cell line HEK 293 shows localization to nucleus.](http://images.novusbio.com/fullsize/KDEL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57105-img0001.jpg "Immunocytochemistry/Immunofluorescence: KDEL Antibody [NBP2-57105] - Staining of human cell line HEK 293 shows localization to nucleus.")
![Immunocytochemistry/Immunofluorescence: KDEL Antibody [NBP2-55448] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KDEL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55448-img0001.jpg "Immunocytochemistry/Immunofluorescence: KDEL Antibody [NBP2-55448] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")