Name: KDEL Antibody (10C3)
Brand: Novus Biologicals
SKU: NBP1-97469
Rating: 4.5 (2 reviews)

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reviewed by:
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Human

10/31/2020

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Summary

Application	Western Blot
Sample Tested	Human primary fibroblast
Species	Human
Lot	08071825

Comments

Primary antibody 1:1000 incubation O/N 4ºC in Milk 5% in PBST (0.1% Tween)

ICC

02/25/2020

Application	Immunocytochemistry
Sample Tested	Human primary fibroblast
Species	Human
Lot	08071825

Great ab for ICC-IF in human fibroblasts. Ab dilution 1:600 (higher dilution needed) in PBST (0.1% triton) + 10% DS. O/N incubation at 4ºC. Secondary Donkey anti-mouse Alexa fluor 488 1:500 in PBST (0.1% triton) + 10% DS 1h RT.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Av, Ch, In, Ma, Pl, Pm, YeSpecies Glossary
Applications	WB, ELISA, Flow, ICC/IF, IHC, IP, MS
Clone	10C3
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Immunogen	Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
Marker	Endoplasmic Reticulum Marker
Isotype	IgG2a
Clonality	Monoclonal
Host	Mouse
Gene	POGLUT2
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	PBS (pH 7.2), 50% Glycerol
Preservative	0.09% Sodium Azide
Purity	Protein G purified

Publications using NBP1-97469	Applications	Species
Song Z, Thepsuwan P, Hur WS et al. Regulation of hepatic inclusions and fibrinogen biogenesis by SEL1L-HRD1 ERAD Nature Communications 2024-10-26 [PMID: 39455574]
Xiaoqiong Wei, You Lu, Liangguang Leo Lin, Chengxin Zhang, Xinxin Chen, Siwen Wang, Shuangcheng Alivia Wu, Zexin Jason Li, Yujun Quan, Shengyi Sun, Ling Qi Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis. Nature communications 2024-01-24 [PMID: 38253565]
Wu, SA;Shen, C;Wei, X;Zhang, X;Wang, S;Chen, X;Torres, M;Lu, Y;Lin, LL;Wang, HH;Hunter, AH;Fang, D;Sun, S;Ivanova, MI;Lin, Y;Qi, L; The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes Nature communications 2023-05-30 [PMID: 37253728] (Immunocytochemistry/ Immunofluorescence, Mouse)	Immunocytochemistry/ Immunofluorescence	Mouse
Jacquier A, Risson V, Simonet T et al. Severe congenital myasthenic syndromes caused by agrin mutations affecting secretion by motoneurons Acta neuropathologica 2022-08-10 [PMID: 35948834] (ICC/IF) Details: Dilutions: 1:400	ICC/IF
Maddineni P, Kasetti RB, Kodati B et al. Sodium 4-Phenylbutyrate Reduces Ocular Hypertension by Degrading Extracellular Matrix Deposition via Activation of MMP9 International journal of molecular sciences 2021-09-18 [PMID: 34576258] (ICC/IF, Mouse)	ICC/IF	Mouse
Coudert L, Osseni A, Gangloff Y Et Al. The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways BMC Biology 2021-12-01 [PMID: 34330273] (ICC/IF)	ICC/IF
Wang G, Xu J, Zhao J et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice Nat Commun 2020-01-10 [PMID: 31924786] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Chang MH, Brown MR, Liu Y et al. Cl- and H+ coupling properties and subcellular localizations of wildtype and disease-associated variants of the voltage-gated Cl-/H+ exchanger ClC-5 J. Biol. Chem. 2019-12-18 [PMID: 31852738] (ICC/IF, Human)	ICC/IF	Human
Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep 2019-02-07 [PMID: 30723259] (IHC-P, Mouse)	IHC-P	Mouse
Hao Y, Chow AW, Yip WC et al. G protein-coupled estrogen receptor inhibits the P2Y receptor-mediated Ca(2+) signaling pathway in human airway epithelia. Pflugers Arch. 2016-06-06 [PMID: 27271044] (ICC/IF, Human)	ICC/IF	Human
Kilaparty SP, Agarwal R, Singh P et al. Endoplasmic reticulum stress-induced apoptosis accompanies enhanced expression of multiple inositol polyphosphate phosphatase 1 (Minpp1): a possible role for Minpp1 in cellular stress response. Cell Stress Chaperones. 2016-04-02 [PMID: 27038811] (WB, Mouse) Details: KDEL monoclonal antibody was used for WB analysis of microsomal and cytosolic fractions of MC3T3-E1 cells which were incubated or not with 100 uM etoposide for 24 hours. The antibody detected 75 and 58 kDa bands - the 75 kDa protein was distributed in b	WB	Mouse
S Tan et al. Nedd4 Family-interacting Protein 1 (Ndfip1) Is Required for the Exosomal Secretion of Nedd4 Family Proteins. J. Biol. Chem. 283, 32621 . 2008-01-01 [PMID: 18819914] (ICC/IF, Human)	ICC/IF	Human
D Sitterlin et al. The Hepatitis B Virus Precore Protein Is Retrotransported from Endoplasmic Reticulum (ER) to Cytosol through the ER-associated Degradation Pathway. J. Biol. Chem. 283, 32352 . 2008-01-01 [PMID: 18805786] (IP, Human)	IP	Human
Kurosawa K, Nakano M, Yokoseki I et al. ncBAF, a chromatin remodeler, enhances PXR-mediated transcriptional activation in the human and mouse liver bioRxiv 2023-02-05
Muroi H, Hori K, Tokutake Y et al. Oleuropein suppresses mitochondrial reactive oxygen species generation possibly via an activation of transient receptor potential V1 and sirtuin-1 in cultured chicken muscle cells Animal science journal = Nihon chikusan Gakkaiho 2022-01-14 [PMID: 35029000] (ICC/IF, Chicken)	ICC/IF	Chicken
Lane 1: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked). [PMID: 18755873]
Y Saga et al. Pofut1 is required for the proper localization of the Notch receptor during mouse development. Mech. Dev. 125, 663. 2008-01-01 [PMID: 18547789] (ICC/IF, Mouse)	ICC/IF	Mouse
D Ito et al. Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases. Ann. Neurol. 61, 237. 2007-01-01 [PMID: 17387721] (WB, Human)	WB	Human
B Ulmasov et al. Tissue and subcellular distribution of CLIC1. BMC Cell Biol. 8, 8. 2007-01-01 [PMID: 17326840]
HM Prehn et al. The amyloid precursor protein protects PC12 cells against endoplasmic reticulum stress-induced apoptosis. J. Neurochem. 87, 248. 2003-01-01 [PMID: 12969271] (WB, Rat)	WB	Rat
RC Austin et al. Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity. J. Biol. Chem. 278, 17438. 2003-01-01 [PMID: 12621026] (WB, Human)	WB	Human
J Culi & RS Mann. Boca, an endoplasmic reticulum protein required for wingless signaling and trafficking of LDL receptor family members in Drosophila. Cell 112, 343. 2003-01-01 [PMID: 12581524]
Serwold T, Gonzalez F, Kim J. ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum. Nature 419, 480. 2002-01-01 [PMID: 12368856] (WB, ICC/IF, Primate)	WB, ICC/IF	Primate
AS Robinson et al. Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae. Biotechnol. Prog. 18, 942. 2002-01-01 [PMID: 12363344] (WB, Yeast)	WB	Yeast
SE Dryer et al. Developmental regulation of neuronal K(Ca) channels by TGFbeta1: an essential role for PI3 kinase signaling and membrane insertion. J. Neurophysiol. 88, 954. 2002-01-01 [PMID: 12163544] (ICC/IF, Chicken)	ICC/IF	Chicken
R Finnern et al. Efficacy of plant-produced recombinant antibodies against HCG. Hum. Reprod. 17, 2054. 2002-01-01 [PMID: 12151436] (WB, Plant)	WB	Plant
WC Wigley et al. A protein sequence that can encode native structure by disfavoring alternate conformations. Nat. Struct. Biol. 9, 381. 2002-01-01 [PMID: 11938353]
K Toyooka et al. Mass transport of proform of a KDEL-tailed cysteine proteinase (SH-EP) to protein storage vacuoles by endoplasmic reticulum-derived vesicle is involved in protein mobilization in germinating seeds. J. Cell Biol. 148, 453. 2000-01-01 [PMID: 10662772]
VI Titorenko & RA Rachubinski. Mutants of the yeast Yarrowia lipolytica defective in protein exit from the endoplasmic reticulum are also defective in peroxisome biogenesis. Mol. Cell Biol. 18, 2789. 1998-01-01 [PMID: 9566898]
A Choukhi et al. Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins. J. Virol. 72, 3851. 1998-01-01 [PMID: 9557669]
AA Scheel & HR Pelham. Identification of amino acids in the binding pocket of the human KDEL receptor. J. Biol. Chem. 273, 2467. 1998-01-01 [PMID: 9442098]
D Vaux et al. Identification by anti-idiotype antibodies of an intracellular membrane protein that recognizes a mammalian endoplasmic reticulum retention signal. Nature 345, 495. 1990-01-01 [PMID: 2161500]
HR Pelham. The retention signal for soluble proteins of the endoplasmic reticulum. Trends Biochem. Sci. 15, 483. 1990-01-01 [PMID: 2077689]
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KDEL Antibody (10C3)

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