JDP2 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

JDP2 is a gene which codes for a protein that helps to repress transactivation as well as controlling responses such as apoptosis, tumorigenesis, cell differentiation, and antitumogeneris via the modification of histones along with chromatin assembly and is comprised of 163 amino acids, weighs approximately 19 kDa. Studies have been conducted on diseases and disorders relating to this gene including intracranial aneurysms, keratitis, carcinoma, rhabdomyosarcoma, pancreatitis, leukemia, and skeletal muscle neoplasm. JDP2 has also been shown to have interactions with JUND, JUNB, JUN, PGR, and EEF1G in pathways such as the ATF-2 transcription factor and tacrolimus/cyclosporine pathways.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-12101

• JDP2 Antibodies
• JDP2 Lysates
• JDP2 Proteins