ATF3 Antibody

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 9 applications: B/N, Chemotaxis, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, In Vivo, WB.

Showing Publications 1 - 10 of 26. Show All 26 Publications. Collapse Publications.

Publications using NBP1-85816	Applications	Species
Lou Y, Song F, Kang Y, Xu Y Periodic Mechanical Stress Inhibits the Development of Osteoarthritis via Regulating ATF3-Akt Axis Journal of inflammation research 2023-11-28 [PMID: 38046403]
Wang HC, Cheng KI, Tseng KY et al. AAV-glycine receptor ?3 alleviates CFA-induced inflammatory pain by downregulating ERK phosphorylation and proinflammatory cytokine expression in SD rats Molecular medicine (Cambridge, Mass.) 2023-02-15 [PMID: 36792984] (IHC, WB)	IHC, WB
Feng R, Muraleedharan Saraswathy V, Mokalled MH, Cavalli V. Self-renewing macrophages in dorsal root ganglia contribute to promote nerve regeneration Proceedings of the National Academy of Sciences 2023-02-14 [PMID: 36763532]
Xu Y, Li Y, Jadhav K, et al. Hepatocyte ATF3 protects against atherosclerosis by regulating HDL and bile acid metabolism Nature metabolism 2021-01-01 [PMID: 33462514]
Konopleva M, Yap T, Daver N et al. Targeting Oxidative Phosphorylation with a Mitochondrial Complex I Inhibitor is limited by Mechanism-based Toxicity Research Square (IHC-Fr, Mouse)	IHC-Fr	Mouse
Dang I, Brazzo JA, Bae Y, Assoian RK Key role for Rac in the early transcriptional response to ECM stiffness and the stiffness-dependent repression of ATF3 Journal of cell science 2023-09-22 [PMID: 37737020] (WB, Mouse)	WB	Mouse
Yap TA, Daver N, Mahendra M et al. Complex I inhibitor of oxidative phosphorylation in advanced solid tumors and acute myeloid leukemia: phase I trials Nature medicine 2023-01-01 [PMID: 36658425] (IHC-P, Mouse) Details: Dilution used 1:500	IHC-P	Mouse
Cervia L, Shibue T, Borah A et al. Data from A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas American Association for Cancer Research 2023-04-04 [PMID: 36576405] (In Vivo, B/N)	In Vivo, B/N
James NE, Woodman M, De La Cruz P et al. Adaptive transcriptomic and immune infiltrate responses in the tumor immune microenvironment following neoadjuvant chemotherapy in high grade serous ovarian cancer reveal novel prognostic associations and activation of pro-tumorigenic pathways Frontiers in Immunology 2022-09-05 [PMID: 36131935] (IHC)	IHC
Palazzo I, Todd LJ, Hoang TV et al. NFkB-signaling promotes glial reactivity and suppresses M�ller glia-mediated neuron regeneration in the mammalian retina Glia 2022-07-01 [PMID: 35388544]
Quiroz-Figueroa K, Vitali C, Conlon DM Et al. TRIB1 regulates LDL metabolism through CEBP alpha-mediated effects on the LDL receptor in hepatocytes The Journal of clinical investigation 2021-11-15 [PMID: 34779419] (WB, Mouse)	WB	Mouse
Chen Z, Zhang J, Wei D et al. GCN2 Regulates ATF3-p38 MAPK Signaling Transduction in Pulmonary Veno-Occlusive Disease Journal of cardiovascular pharmacology and therapeutics 2021-05-14 [PMID: 33988041]
Zhu X, Xie W, Zhang J et al. Sympathectomy decreases pain behaviors and nerve regeneration by downregulating monocyte chemokine CCL2 in dorsal root ganglia in the rat tibial nerve crush model Pain 2022-01-01 [PMID: 33941753]
Lindborg J, Tran N, Chenette D et al. Optic nerve regeneration screen identifies multiple genes restricting adult neural repair Cell Reports 2021-03-01 [PMID: 33657370] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Ewan EE, Carlin D, Goncalves TM et al. Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism Sci Rep 2021-01-12 [PMID: 33431991]
Kampanis V, Tolou-Dabbaghian B, Zhou L, et al. Cyclic Stretch of Either PNS or CNS Located Nerves Can Stimulate Neurite Outgrowth Cells 2020-12-28 [PMID: 33379276] (Rat)		Rat
Hong L, Li F, Tang C et al. Semaphorin 7A promotes endothelial to mesenchymal transition through ATF3 mediated TGF- beta 2/Smad signaling Cell Death Dis 2020-01-01 [PMID: 32826874] (Chemotaxis, Human)	Chemotaxis	Human
Nguyen TTT, Zhang Y, Shang E et al. Inhibition of HDAC1/2 Along with TRAP1 Causes Synthetic Lethality in Glioblastoma Model Systems Cells 2020-07-10 [PMID: 32664214] (WB, Human)	WB	Human
Vysokov N, McMahon SB, Raouf R The role of NaV channels in synaptic transmission after axotomy in a microfluidic culture platform Sci Rep [PMID: 31501450] (ICC/IF, Rat)	ICC/IF	Rat
Holland, SD;Ramer, LM;McMahon, SB;Denk, F;Ramer, MS; An ATF3-CreERT2 Knock-In Mouse for Axotomy-Induced Genetic Editing: Proof of Principle eNeuro 2019-04-09 [PMID: 30993183] (IHC-Fr, ICC/IF, Mouse)	IHC-Fr, ICC/IF	Mouse
Bianchetti E, Bates SJ, Carroll SL et al. Usp9X Regulates Cell Death in Malignant Peripheral Nerve Sheath Tumors. Sci Rep. 2018-11-26 [PMID: 30478285] (WB, Human)	WB	Human
Mousseau M, Burma NE, Lee KY et al. Microglial pannexin-1 channel activation is a spinal determinant of joint pain Sci Adv 2018-08-01 [PMID: 30101191] (IHC-P, Rat)	IHC-P	Rat
Edagawa M, Kawauchi J, Hirata M et al. Role of Activating Transcription Factor 3 (ATF3) in Endoplasmic Reticulum (ER) Stress-induced Sensitization of p53-deficient Human Colon Cancer Cells to Tumor Necrosis Factor (TNF)-related Apoptosis-inducing Ligand (TRAIL)-mediated Apoptosis through Up-regulation of Death Receptor 5 (DR5) by Zerumbone and Celecoxib. J Biol Chem 2014-08-01 [PMID: 24939851] (WB, Human)	WB	Human
Wei S, Wang H, Lu C et al. The Activating Transcription Factor 3 Protein Suppresses the Oncogenic Function of Mutant p53 Proteins. J Biol Chem 2014-03-28 [PMID: 24554706] (IF/IHC, Human)	IF/IHC	Human
Hai T, Jalgaonkar S, Wolford CC, Yin X. Immunohistochemical Detection of Activating Transcription Factor 3, a Hub of the Cellular Adaptive-Response Network. Methods Enzymol 2011-01-01 [PMID: 21266251]
Wu X, Nguyen BC, Dziunycz P et al. Opposing roles for calcineurin and ATF3 in squamous skin cancer. Nature 2010-05-01 [PMID: 20485437]
Show All 26 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Felix Oppel	Indirect Immunofluorescence	Human	05/03/2022	View Summary Application Other Sample Tested Cell culture Species Human Lot 000016781
Enlarge	4 reviewed by: Verified Customer	IHC-Fr	Feline	10/19/2018	View Summary Application Immunohistochemistry-Frozen Sample Tested Optic nerve Species Feline Lot B114876
Enlarge	5 reviewed by: Joanne Steinauer	IHC-Fr	Rat	09/15/2017	View Summary Application Immunohistochemistry-Frozen Sample Tested Dorsal root ganglion Species Rat Lot B106628 Comments Comments Fixed frozen rat DRG sectioned at 10um and slide mounted. IHC of ATF3 (1:500) incubated overnight at RT after 1 hour blocking. Secondary incubation at RT for 1 hour using Alexa 488 goat anti-rabbit IgG at 1:1000. Snapshot taken with epifluorescent microscope at 20x. Concurrently stained contralateral image (not shown) showed no ATF3 immunoreactivity.

Array NBP1-85816

• ATF3 Antibodies
• ATF3 ELISA Kit
• ATF3 Lysates
• ATF3 Proteins