![Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - Azide and BSA Free [NBP2-47744] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling IDH1. Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1/1152) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-IDH1-1152-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47744-img0004.jpg "Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - Azide and BSA Free [NBP2-47744] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling IDH1. Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1/1152) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clone | IDH1/1152 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45156). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant fragment of human Isocitrate Dehydrogenase 1/IDH1 protein (around aa 281-414) (exact sequence is proprietary) (Uniprot: O75874 ) |
| Localization | Cytoplasmic & Nuclear |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IDH1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 1mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP2-47744)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IDH1 |
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