I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

Name: Integrin beta 1/CD29 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF2405
Price: 479 USD

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I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Mu, RtSpecies Glossary
Applications	WB, Simple Western, Flow, IHC, CyTOF-ready
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF2405

Catalog# & Formulation	Size	Price
Carrier Free AF2405-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2405

Integrin beta 1/CD29 Antibody [Unconjugated]

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Immunogen	Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1 Gln21-Ala738 Accession # P09055
Specificity	Detects mouse Integrin beta 1/CD29 in direct ELISAs. Detects mosue and rat Integrin beta 1/CD29 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 1 and rhIntegrin beta 3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 4, and rmIntegrin beta 6 is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	ITGB1
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF2405	Applications	Species
Agnello, L;d'Argenio, A;Caliendo, A;Nilo, R;Zannetti, A;Fedele, M;Camorani, S;Cerchia, L; Tissue Inhibitor of Metalloproteinases-1 Overexpression Mediates Chemoresistance in Triple-Negative Breast Cancer Cells Cells 2023-07-07 [PMID: 37443843] (Western Blot, Rat)	Western Blot	Rat
Y Xue, C Lyu, A Taylor, A Van Ee, A Kiemen, Y Choi, N Khavanian, D Henn, C Lee, L Hwang, E Wier, S Wang, S Lee, A Li, C Kirby, G Wang, PH Wu, D Wirtz, LA Garza, SK Reddy Mechanical tension mobilizes Lgr6+ epidermal stem cells to drive skin growth Science Advances, 2022-04-27;8(17):eabl8698. 2022-04-27 [PMID: 35476447] (IHC/IF, Mouse)	IHC/IF	Mouse
A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis Cell Reports, 2021-10-26;37(4):109885. 2021-10-26 [PMID: 34706238] (IHC, Mouse)	IHC	Mouse
Changshui Wang, Cuilian Sun, Yueshu Zhao, Huimin Song, Zhengyou Li, Feng Jin et al. RNF213 gene silencing upregulates transforming growth factor beta 1 expression in bone marrow‑derived mesenchymal stem cells and is involved in the onset of Moyamoya disease Experimental and Therapeutic Medicine 7/15/2021 [PMID: 34373710]
Jessica Cristina Marín-Llera, Carlos Ignacio Lorda-Diez, Juan Mario Hurle, Jesús Chimal-Monroy SCA-1/Ly6A Mesodermal Skeletal Progenitor Subpopulations Reveal Differential Commitment of Early Limb Bud Cells Frontiers in Cell and Developmental Biology 7/16/2021 [PMID: 34336823]
Argelia S. García-Cervera, Jesús Chimal-Monroy, Jessica C. Marín-llera The spatiotemporal expression patterns of MSC-associated markers contribute to the identification of progenitor subpopulations in developing limbs The International Journal of Developmental Biology 12/18/2020 [PMID: 33336711]
Georgia Nikoloudaki, Paige Snider, Olga Simmons, Simon. J. Conway, Douglas W. Hamilton Periostin and matrix stiffness combine to regulate myofibroblast differentiation and fibronectin synthesis during palatal healing Matrix Biology 12/1/2020 [PMID: 32777343]
S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020-08-04;27(3):396-412.e6. 2020-08-04 [PMID: 32755548] (Flow Cytometry, IHC, Transgenic Mouse)	Flow Cytometry, IHC	Transgenic Mouse
Chao Liu, Jiao Sun Modulation of the secretion of mesenchymal stem cell immunoregulatory factors by hydrolyzed fish collagen Experimental and Therapeutic Medicine 4/22/2020 [PMID: 32509014]
A Heinrich, SJ Potter, L Guo, N Ratner, T DeFalco Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis Cell Rep, 2020-04-14;31(2):107513. 2020-04-14 [PMID: 32294451] (IF, Mouse)	IF	Mouse
Sarah J. Parker, Aleksandr Stotland, Elena MacFarlane, Nicole Wilson, Amanda Orosco, Vidya Venkatraman et al. Proteomics reveals Rictor as a noncanonical TGF-beta signaling target during aneurysm progression in Marfan mice American Journal of Physiology-Heart and Circulatory Physiology 11/1/2018 [PMID: 30004239]
Minlong Zhang, Xue Yan, Wei Liu, Ruilin Sun, Yonghong Xie, Faguang Jin Endothelial semaphorin 7A promotes seawater aspiration-induced acute lung injury through plexin C1 and beta 1 integrin Molecular Medicine Reports 10/1/2017 [PMID: 28765893]
UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 2017-02-20;19(3):189-201. 2017-02-20 [PMID: 28218908] (IHC, Mouse)	IHC	Mouse
Wu , B, Cloer , C, Lu , P, Milazi , S, Shaban , M, Shah , S N, Marston-Poe , L, Moulton , H M, Lu , Q L Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice. Gene Ther, 2014-06-19;21(9):785-93. 2014-06-19 [PMID: 24942628] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5. J. Neurochem., 2011-01-19;118(4):521-32. 2011-01-19 [PMID: 21166806] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
Liu S, Kapoor M, Denton CP, Abraham DJ, Leask A Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model. Arthritis Rheum., 2009-09-01;60(9):2817-21. 2009-09-01 [PMID: 19714619] (ICC, Western Blot, Mouse)	ICC, Western Blot	Mouse
Gendronneau G, Sidhu SS, Delacour D, Dang T, Calonne C, Houzelstein D, Magnaldo T, Poirier F Galectin-7 in the control of epidermal homeostasis after injury. Mol. Biol. Cell, 2008-10-01;19(12):5541-9. 2008-10-01 [PMID: 18829868] (IHC, Mouse)	IHC	Mouse
Chang Y, Finnemann SC Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis. J. Cell. Sci., 2007-08-07;120(0):3053-63. 2007-08-07 [PMID: 17684062] (ICC, Immunoprecipitation, Rat)	ICC, Immunoprecipitation	Rat
Show All 18 Publications. Collapse Publications.

Gene Symbol	ITGB1
Uniprot	P09055()