Home » IL-7R alpha/CD127 » IL-7R alpha/CD127 Antibodies » IL-7R alpha/CD127 Antibody [Phycoerythrin]

IL-7R alpha/CD127 Antibody [Phycoerythrin]

 (1 Publication)
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Rat splenocytes were stained with anti-rat CD4 APC-conjugated antibody and either (A) Sheep Anti-Rat IL-7 Ra/CD127 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5607P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.
Rat splenocytes were stained with anti-rat CD4 APC-conjugated antibody and either (A) Sheep Anti-Rat IL-7 Ra/CD127 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5607P) or (B) Normal Sheep IgG ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF5607
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF5607
Carrier Free
AF5607
Carrier Free
AF5607-SP
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IL-7R alpha/CD127 Antibody [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha /CD127
Glu21-Asp239
Accession # NP_001099888
Specificity
Detects rat IL-7 R alpha /CD127 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IL-7 R alpha and less than 10% cross-reactivity with recombinant human IL-7 R alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
IL7R
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
FAB5607P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-7R alpha/CD127 Antibody [Phycoerythrin]

  • CD127 antigen
  • CD127
  • CD127ILRA
  • CDW127
  • IL-7 R alpha
  • IL-7 receptor subunit alpha
  • IL7R alpha
  • IL-7R alpha
  • IL-7R subunit alpha
  • IL7R
  • IL7RA
  • IL-7Ra
  • IL-7R-alpha
  • interleukin 7 receptor alpha chain
  • interleukin 7 receptor isoform H5-6
  • interleukin 7 receptor
  • interleukin-7 receptor subunit alpha

Background

IL-7 R alpha (IL-7 Receptor alpha; also known as CD127) is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 amino acid (aa) type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III fibronectin domain (aa 129-222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21-239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-7R alpha/CD127 Antibody (FAB5607P)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol IL7R
Uniprot