| Reactivity | HuSpecies Glossary |
| Applications | Flow |
| Clone | 40131 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Phycoerythrin |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha Extracellular domain |
| Specificity | Detects human IL-7 R alpha in Western blots. In Western blots, approximately 5% cross-reactivity was observed with recombinant human (rh) IL‑10 R, rhIL‑2 R beta , rhIL‑5 R alpha , and rhIL‑6 R. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IL7R |
| Purity Statement | Protein A or G purified from ascites |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Protect from light. Do not freeze.
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| Buffer | Supplied in a saline solution containing BSA and Sodium Azide. |
| Preservative | Sodium Azide |
Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha . IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).
![Western Blot PD-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/PD1_AF1086_Western_Blot_20838.jpg)
![Immunohistochemistry PD-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/PD1_AF1086_Immunohistochemistry_17216.jpg)
![Western Blot PD-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/PD1_AF1086_Western_Blot_19120.jpg)
![Flow Cytometry L-Selectin/CD62L Antibody (4G8) [Unconjugated]](https://images.novusbio.com/images/antibody/bba24_human-l-selectin-cd62l-mab-cl-4g8-flow-cytometry-822024174319..jpg)
![IL-7R alpha/CD127 Antibody (40131) [Phycoerythrin]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-7R alpha/CD127 Antibody (40131) [Phycoerythrin]")
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
Flow | Human | 02/08/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IL7R |
![N/A CD25/IL-2R alpha [HRP]](https://images.novusbio.com/images2/IL-2_R_alpha_DR2A00_ELISA_167.jpg)
![N/A CD25/IL-2R alpha [HRP]](https://images.novusbio.com/images2/DATA_CD25_DR2A00_ELISA_800.jpg)
![SEC-MALS IL-7 [Unconjugated]](https://images.novusbio.com/images/207-il_recombinant-human-il-7-protein-sec-mals-123202493447..jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SEC-MALS IL-15 [Unconjugated]](https://images.novusbio.com/images/247-ilb_recombinant-human-il-15-protein-sec-mals-2922024964..jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Neutralization Common gamma Chain/IL-2 R gamma Antibody [Unconjugated]](https://images.novusbio.com/images2/Common_gamma_Chain_AF284_Block_Neutralize_709.jpg)
