Home » IL-7R alpha/CD127 » IL-7R alpha/CD127 Antibodies » IL-7R alpha/CD127 Antibody (1140A) [CoraFluor™ 1]

IL-7R alpha/CD127 Antibody (1140A) [CoraFluor™ 1]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
1140A
Clonality
Monoclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB7473U-100UG
Alexa Fluor 405
FAB7473V-100UG
Alexa Fluor 488
FAB7473G-100UG
Alexa Fluor 532
FAB7473X
Alexa Fluor 594
FAB7473T-100UG
Alexa Fluor 647
FAB7473R-100UG
Alexa Fluor 700
FAB7473N-100UG
Alexa Fluor 750
FAB7473S-100UG
Allophycocyanin
FAB7473A
Allophycocyanin/Cy7
FAB7473APCCY7
Biotin
FAB7473B
DyLight 350
FAB7473D
DyLight 405
FAB7473E
DyLight 488
FAB7473K
DyLight 550
FAB7473L
DyLight 594
FAB7473M
DyLight 650
FAB7473W
DyLight 680
FAB7473Y
DyLight 755
FAB7473Z
FITC
FAB7473F
HRP
FAB7473H
Janelia Fluor 525
FAB7473JF525
Janelia Fluor 549
FAB7473I
Janelia Fluor 646
FAB7473J
Janelia Fluor 669
FAB7473JF669
PE
FAB7473P
PE/Atto594
FAB7473PEATT594
PE/Cy5.5
FAB7473PECY55
PE/Cy7
FAB7473PECY7
PerCP
FAB7473C
Unconjugated
MAB7473
mFluor Violet 450 SE
FAB7473MFV450
mFluor Violet 500 SE
FAB7473MFV500
mFluor Violet 610 SE
FAB7473MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB7473
Carrier Free
FAB7473R-100UG
Carrier Free
FAB7473G-100UG
Carrier Free
MAB7473
Carrier Free
MAB7473-SP
Carrier Free
FAB7473V-100UG
Carrier Free
FAB7473U-100UG
Carrier Free
FAB7473T-100UG
Carrier Free
FAB7473S-100UG
Carrier Free
FAB7473N-100UG
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IL-7R alpha/CD127 Antibody (1140A) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Recombinant Monoclonal Antibody.
Immunogen
NSO mouse myeloma cell line transfected with mouse IL‑7 R alpha /CD127
Glu21-Asp239
Accession # P16872
Specificity
Stains mouse IL‑7 R alpha /CD127 transfectants but not irrelevant transfectants in flow cytometry.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IL7R
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for IL-7R alpha/CD127 Antibody (1140A) [CoraFluor™ 1]

  • CD127 antigen
  • CD127
  • CD127ILRA
  • CDW127
  • IL-7 R alpha
  • IL-7 receptor subunit alpha
  • IL7R alpha
  • IL-7R alpha
  • IL-7R subunit alpha
  • IL7R
  • IL7RA
  • IL-7Ra
  • IL-7R-alpha
  • interleukin 7 receptor alpha chain
  • interleukin 7 receptor isoform H5-6
  • interleukin 7 receptor
  • interleukin-7 receptor subunit alpha

Background

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL7R