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IL-27 Antibody

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Western Blot: IL-27 Antibody [NB100-56389] - Analysis of IL-27 in A) Jurkat and B) NIH 3T3 cells using this antibody at 1 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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IL-27 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 31-46 (RPPGRPQLSLQELRRE) of human IL-27 (p28 subunit) of Genbank accession number NP_663634 was used as immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL27
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 21294722)
  • Western Blot 1-2 ug/ml
Publications
Read Publication using
NB100-56389 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for IL-27 Antibody

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A

Background

Recently, a new heterodimeric cytokine termed IL-27 that consists of EBI3 (Epstein-Barr virus-induced gene 3), an IL-12p40-related protein, and p28, a newly discovered IL-12p35-related polypeptide has been discovered. IL-27 is involved in early Th1 initiation. Its action is mediated through the orphan cytokine receptor WSX-1/TCCR and gp130. IL-27 may also play a role in the induction of T-bet expression and regulation of Ig class switching in B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-27 Antibody (NB100-56389)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IL-27 Antibody (NB100-56389) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for IL-27 Antibody (NB100-56389)

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Blogs on IL-27.

Ly6G Antibody - A Marker for Monocytes, Granulocytes and Neutrophils
What is Ly6G?Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. M...  Read full blog post.

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Bioinformatics

Gene Symbol IL27
Entrez
Uniprot