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IFN-gamma R2 Antibody [Unconjugated]

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Blood-derived granulocytes were stained with Goat Anti-Human IFN‑ gamma R2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF773, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Blood-derived granulocytes were stained with Goat Anti-Human IFN‑ gamma R2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF773, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), ...read more
Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). ...read more
B3GNT2 disrupts ligand–receptor interactions between tumor and T cells.a Cell survival against T cell cytotoxicity (top) and T cell IFN gamma secretion (bottom) in A375 cells overexpressing B3GNT2 or GFP that have ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
FAB773A
Biotin
BAF773
Fluorescein
FAB773F
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB773A
Carrier Free
FAB773F
Carrier Free
AF773-SP
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IFN-gamma R2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2
Ala22-Gln247
Accession # P38484
Specificity
Detects human IFN-gamma  R2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFNGR2
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF773 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma R2 Antibody [Unconjugated]

  • AF-1
  • AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1
  • IFGR2
  • IFN-gamma R2
  • IFN-gamma receptor 2
  • IFNgammaR2
  • IFN-gamma-R2
  • IFNGR2
  • IFN-gR2
  • IFNGT1
  • interferon gamma receptor 2 (interferon gamma transducer 1)
  • interferon gamma receptor 2
  • interferon gamma receptor accessory factor-1
  • interferon gamma receptor beta chain
  • Interferon gamma transducer 1

Background

Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma . IFN‑ gamma  R2 is required for signal transduction but does not bind IFN-gamma by itself.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IFN-gamma R2
Antibody [Unconjugate
AF773
IFN-gamma R2 Antibody [Unconjugated]
Species: Hu
Applications: WB, Flow, B/N, CyTOF-ready

Publications for IFN-gamma R2 Antibody (AF773)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


Filter By Application
Flow Cytometry
(2)
Western Blot
(3)
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Human
(5)
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Showing Publications 1 - 5 of 5.
Publications using AF773 Applications Species
Khanolkar, A;Liu, G;Simpson Schneider, BM; Defining the Basal and Immunomodulatory Mediator-Induced Phosphoprotein Signature in Pediatric B Cell Acute Lymphoblastic Leukemia (B-ALL) Diagnostic Samples International journal of molecular sciences 2023-09-11 [PMID: 37762241] (Flow Cytometry, Human) Flow Cytometry Human
J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022-03-25;13(1):1606. 2022-03-25 [PMID: 35338135] (Western Blot, Human) Western Blot Human
CM Blouin, Y Hamon, P Gonnord, C Boularan, J Kagan, C Viaris de, R Ruez, S Mailfert, N Bertaux, D Loew, C Wunder, L Johannes, G Vogt, FX Contreras, D Marguet, JL Casanova, C Galès, HT He, C Lamaze Glycosylation-Dependent IFN-?R Partitioning in Lipid and Actin Nanodomains Is Critical for JAK Activation Cell, 2016-08-04;166(4):920-34. 2016-08-04 [PMID: 27499022] (Western Blot, Human) Western Blot Human
Trisomy 21 consistently activates the interferon response Elife, 2016-07-29;5(0):. 2016-07-29 [PMID: 27472900] (Western Blot, Human) Western Blot Human
Lee WI, Liang FC, Huang JL, Jaing TH, Wang CH, Lin TY, Huang YC, Huang WL, Jou R, Hsieh MY, Chia JH, Wu TL Immunologic analysis of HIV-uninfected Taiwanese children with BCG-induced disease. J. Clin. Immunol., 2008-12-13;29(3):319-29. 2008-12-13 [PMID: 19089604] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol IFNGR2
Uniprot