ICAM-3/CD50 Antibody Summary
Immunogen |
Chinese hamster ovary cell line CHO-derived recombinant human ICAM‑3/CD50 |
Specificity |
Detects human ICAM‑3/CD50 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) ICAM-1 is observed and less than 1% cross-reactivity with recombinant mouse (rm) ICAM-1, recombinant rat ICAM-1, rhICAM-2, rhVCAM-1, and rmVCAM-1 is observed. |
Source |
N/A |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Sheep |
Gene |
ICAM3 |
Purity Statement |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- CyTOF-ready
- Flow Cytometry 2.5 ug/10^6 cells
- Western Blot 0.1 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Reconstitution Instructions |
Reconstitute at 0.2 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ICAM-3/CD50 Antibody
Background
Intercellular Adhesion Molecule‑3 (ICAM‑3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA‑1 (CD11a/CD18) and alpha d/ beta 2. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM‑3 has not been identified in the mouse.
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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