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HspA4L Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: HspA4L Antibody [NBP2-48700] - Staining in human testis and endometrium tissues using anti-HSPA4L antibody. Corresponding HSPA4L RNA-seq data are presented ...read more
Western Blot: HspA4L Antibody [NBP2-48700] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: HspA4L Antibody [NBP2-48700] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Immunohistochemistry-Paraffin: HspA4L Antibody [NBP2-48700] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: HspA4L Antibody [NBP2-48700] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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HspA4L Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KCHAEHTPEEEIDHTGAKTKSAVSDKQDRLNQTLKKGKVKSIDLPIQSSLCRQLGQDLLNSY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA4L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
HspA4L Recombinant Protein Antigen (NBP2-48700PEP)

Reactivity Notes

Mouse (85%), Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HspA4L Antibody

  • APG1
  • APG-1
  • heat shock 70 kDa protein 4L
  • heat shock 70 kDa protein 4-like protein
  • heat shock 70kDa protein 4-like
  • Heat shock 70-related protein APG-1
  • heat shock protein (hsp110 family)
  • Osmotic stress protein 94
  • Osp94

Background

HSPA4L is a novel autocrine pathway of CD1d regulation by Hsp110.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HSPA4L