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HSD17B3 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: HSD17B3 Antibody [NBP2-38807] - Analysis in human testis and skin tissues using NBP2-38807 antibody. Corresponding HSD17B3 RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: HSD17B3 Antibody [NBP2-38807] - Staining of human skin shows negative cytoplasmic positivity in keratinocytes as expected.
Immunohistochemistry-Paraffin: HSD17B3 Antibody [NBP2-38807] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: HSD17B3 Antibody [NBP2-38807] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: HSD17B3 Antibody [NBP2-38807] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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HSD17B3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NVGILPNLLPSHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQKGLIL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSD17B3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
HSD17B3 Protein (NBP2-38807PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HSD17B3 Antibody

  • EC 1.1.1.64
  • EDH17B317-beta-HSD3
  • hydroxysteroid (17-beta) dehydrogenase 3,17-beta-hydroxysteroid dehydrogenase type 3
  • SDR12C2
  • short chain dehydrogenase/reductase family 12C, member 2
  • Testicular 17-beta-hydroxysteroid dehydrogenase
  • testosterone 17-beta-dehydrogenase 3,17-beta-HSD 3

Background

HSD17B3 is an isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSD17B3
Uniprot