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17 beta-HSD1/HSD17B1 Antibody [Unconjugated]

 
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Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human 17 beta -HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for 17 beta -HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human 17 beta -HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) followed by ...read more
17 beta ‑HSD1/HSD17B1 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human 17 beta ‑HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody ...read more
Simple Western lane view shows lysates of human placenta, loaded at 0.2 mg/mL. A specific band was detected for 17 beta ‑HSD1/HSD17B1 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF7178-SP
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17 beta-HSD1/HSD17B1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human 17 beta -HSD1/HSD17B1
Ala2-Gln328
Accession # P14061
Specificity
Detects human 17 beta -HSD1/HSD17B1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
HSD17B1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2.5 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 17 beta-HSD1/HSD17B1 Antibody [Unconjugated]

  • 17 betaHSD1
  • 17 beta-HSD1
  • 17-beta-HSD 1
  • 17-beta-hydroxysteroid dehydrogenase type 1
  • 20 alpha-hydroxysteroid dehydrogenase
  • 20-alpha-HSD
  • E17KSR
  • E2DH
  • EC 1.1.1.62
  • EDH17B1
  • EDH17B2
  • EDH17B2EDHB17
  • EDHB17
  • estradiol 17-beta-dehydrogenase 1
  • estradiol 17-beta-dehydrogenase-1
  • HSD17
  • HSD17B1
  • hydroxysteroid (17-beta) dehydrogenase 1 isoform
  • hydroxysteroid (17-beta) dehydrogenase 1
  • MGC138140
  • Placental 17-beta-hydroxysteroid dehydrogenase
  • SDR28C1
  • short chain dehydrogenase/reductase family 28CE, member 1

Background

HSD17B1 (Hydroxysteroid 17-beta dehydrogenase type 1; also 17  beta ‑HSD1 and E2DH) is a 33-34 kDa member of the short chain dehydrogenase/reductase (SDR) superfamily of molecules. It is a cytoplasmic breast, placental and gonadal enzyme that both converts DHEA to estrogen A-diol, and inactivates DHT through oxidation and reduction. Human HSD17B1 is 328 amino acids (aa) in length. It contains an NADP binding site (aa 10-38) and a steroid catalytic site (aa 210-221). HSD17B1 functions as a 68 kDa noncovalently-linked homodimer. Over aa 2-328, human HSD17B1 shares 65% aa identity with mouse HSD17B1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HSD17B1
Uniprot