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HSD17B2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: HSD17B2 Antibody [NBP1-92011] - Analysis in human duodenum and testis tissues. Corresponding HSD17B2 RNA-seq data are presented for the same tissues.
Western Blot: HSD17B2 Antibody [NBP1-92011] - Analysis in control (vector only transfected HEK293T lysate) and HSD17B2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: HSD17B2 Antibody [NBP1-92011] - Staining of human cell line RT4 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HSD17B2 Antibody [NBP1-92011] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: HSD17B2 Antibody [NBP1-92011] - Staining of human urinary bladder shows moderate to strong cytoplasmic positivity in urothelial cells.
Immunohistochemistry-Paraffin: HSD17B2 Antibody [NBP1-92011] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: HSD17B2 Antibody [NBP1-92011] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemistry-Paraffin: HSD17B2 Antibody [NBP1-92011] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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HSD17B2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSD17B2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
HSD17B2 Protein (NBP1-92011PEP)
Publications
Read Publication using NBP1-92011.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HSD17B2 Antibody

  • 17-beta-hydroxysteroid dehydrogenase type 2
  • 20 alpha-hydroxysteroid dehydrogenase
  • E2DH
  • EC 1.1.1.62,17-beta-HSD 2
  • EC 1.1.1.63
  • EDH17B220-alpha-HSD
  • estradiol 17-beta-dehydrogenase 2
  • HSD17
  • hydroxysteroid (17-beta) dehydrogenase 2
  • Microsomal 17-beta-hydroxysteroid dehydrogenase
  • SDR9C2
  • short chain dehydrogenase/reductase family 9C, member 2
  • Testosterone 17-beta-dehydrogenase

Background

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol andestrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSD17B2 Antibody (NBP1-92011)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HSD17B2
Entrez
Uniprot