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HO-1/HMOX1/HSP32 Antibody [Unconjugated]

 (8 Publications)
 
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Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-1/HMOX1/HSP32 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse ...read more
Simple Western lane view shows lysates of A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HO‑1/HMOX1/HSP32 at approximately 37 kDa (as ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3776-SP
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HO-1/HMOX1/HSP32 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HO‑1/HMOX1/HSP32
Met1-Thr261
Accession # P09601
Specificity
Detects human and mouse HO‑1/HMOX1/HSP32 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HO-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HMOX1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Simple Western 25 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF3776 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HO-1/HMOX1/HSP32 Antibody [Unconjugated]

  • bK286B10
  • EC 1.14.99.3
  • heat shock protein, 32-kD
  • heme oxygenase (decycling) 1
  • HMOX1
  • HO
  • HO1
  • HO-1
  • HO-1heme oxygenase 1
  • HSP32

Background

Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HO-1/HMOX1/HSP32 Antibody (AF3776)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Simple Western, Western Blot.


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(1)
Western Blot
(3)
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(2)
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Showing Publications 1 - 8 of 8.
Publications using AF3776 Applications Species
Arisa Senda, Ryutaro Sasai, Kurumi Kato, Yuka Nishibata, Sakiko Masuda, Akihiro Ishizu et al. Involvement of neutrophil extracellular traps in the pathogenesis of glomerulonephritis in a case of systemic lupus erythematosus and antineutrophil cytoplasmic antibody-associated vasculitis overlap syndrome CEN Case Reports 2022-08-01 [PMID: 35025058]
MW Kim, JH Kang, HJ Jung, SY Park, THL Phan, H Namgung, SY Seo, YS Yoon, SH Oh Allyl Isothiocyanate Protects Acetaminophen-Induced Liver Injury via NRF2 Activation by Decreasing Spontaneous Degradation in Hepatocyte Nutrients, 2020-11-23;12(11):. 2020-11-23 [PMID: 33238403] (Western Blot, Mouse) Western Blot Mouse
Sarka Tumova, Michael J. Houghton, Gary Williamson The effect of quercetin on endothelial cells is modified by heterocellular interactions Food & Function 2020-05-01 [PMID: 32363357] (Simple Western) Simple Western
JT Liu, HY Chen, WC Chen, KM Man, YH Chen Red Yeast Rice Protects Circulating Bone Marrow-Derived Proangiogenic Cells against High-Glucose-Induced Senescence and Oxidative Stress: The Role of Heme Oxygenase-1 Oxid Med Cell Longev, 2017-05-06;2017(0):3831750. 2017-05-06 [PMID: 28555162] (Western Blot, Human) Western Blot Human
NIKOLAOS GOUTZOURELAS, DIMITRIOS STAGOS, ANASTASIA HOUSMEKERIDOU, CHRISTINA KARAPOULIOU, EFTHALIA KERASIOTI, NEKTARIOS ALIGIANNIS et al. Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells International Journal of Molecular Medicine 2015-08-01 [PMID: 26082074]
Jing Wang, Arul Shanmugam, Shanu Markand, Eric Zorrilla, Vadivel Ganapathy, Sylvia B. Smith Sigma 1 receptor regulates the oxidative stress response in primary retinal Müller glial cells via NRF2 signaling and system xc(-), the Na(+)-independent glutamate-cystine exchanger Free Radical Biology and Medicine 2015-09-01 [PMID: 25920363]
Jan Fiedler, Andrea Stöhr, Shashi Kumar Gupta, Dorothee Hartmann, Angelika Holzmann, Annette Just et al. Functional MicroRNA Library Screening Identifies the HypoxaMiR MiR-24 as a Potent Regulator of Smooth Muscle Cell Proliferation and Vascularization Antioxidants & Redox Signaling 2014-09-10 [PMID: 24063572]
Tao JY, Zheng GH, Zhao L, Wu JG, Zhang XY, Zhang SL, Huang ZJ, Xiong FL, Li CM Anti-inflammatory effects of ethyl acetate fraction from Melilotus suaveolens Ledeb on LPS-stimulated RAW 264.7 cells. J Ethnopharmacol, 2009-03-03;123(1):97-105. 2009-03-03 [PMID: 19429346] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol HMOX1
Uniprot