HO-1/HMOX1/HSP32 Antibodies
We offer HO-1/HMOX1/HSP32 Antibodies for use in common research applications: Blocking/Neutralizing, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each HO-1/HMOX1/HSP32 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HO-1/HMOX1/HSP32 Antibodies can be used in a variety of model species: Bovine, Canine, Hamster, Human, Mouse, Primate, Rabbit, Rat. Use the list below to choose the HO-1/HMOX1/HSP32 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HO-1/HMOX1/HSP32 polyclonal antibodies and browse our HO-1/HMOX1/HSP32 monoclonal antibody catalog.
Alternate Names for HO-1/HMOX1/HSP32 Antibodies
anti-HO-1/HMOX1/HSP32 antibody, anti-HMOX1 antibody, anti-bK286B10 antibody, anti-EC 1.14.99.3 antibody, anti-heat shock protein, 32-kD antibody, anti-heme oxygenase (decycling) 1 antibody, anti-HO antibody, anti-HO-1heme oxygenase 1 antibody, anti-HO1 antibody, anti-HSP32 antibody
15 Results for "ho-1-hmox1-hsp32" in Primary Antibodies
![Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-HO-1-1-Western-Blot-NBP1-97507-img0004.jpg "Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.")
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10ug/ml.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-HO-1-1-Immunohistochemistry-Paraffin-NBP1-97507-img0003.jpg)
![Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of 10^6 Jurkat cells stained using HO-1 monoclonal antibody (HO-1-1), (Prod. No. ADI-OSA-110), at a concentration of 50 ug/mL](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-HO-1-1-Flow-Cytometry-NBP1-97507-img0005.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Mouse liver. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-31341-img0036.jpg "Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Mouse liver. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Analysis of HO-1 in HEK293 cell lysate using anti-HO-1 antibody. WB Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Western-Blot-NBP1-31341-img0017.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Western-Blot-NBP1-31341-img0022.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:1500.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Western-Blot-NBP1-31341-img0023.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Nrf2-pathway activation by hybrids: nuclear translocation and targets induction. Protein levels by Western blot. Anti-actin was used as protein loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32047434/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Western-Blot-NBP1-31341-img0032.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Human renal carcinoma . Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-31341-img0019.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-31341-img0035.jpg)
![ELISA: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - ELISA detection of recombinant full-length Heme Oxygenase 1 protein using as capture antibody at concentration of 5 ug/mL and as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-ELISA-NBP1-31341-img0030.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Knockout-Validated-NBP1-31341-img0028.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31341_rabbit-polyclonal-ho-1-hmox1-hsp32-antibody-910202420115032.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31341_rabbit-polyclonal-ho-1-hmox1-hsp32-antibody-91020242001087.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - HO-1/HMOX1/HSP32 antibody detects HO-1/HMOX1/HSP32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.HO-1/HMOX1/HSP32 stained by HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31341_rabbit-polyclonal-ho-1-hmox1-hsp32-antibody-910202420104478.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31341_rabbit-polyclonal-ho-1-hmox1-hsp32-antibody-910202420104459.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31341_rabbit-polyclonal-ho-1-hmox1-hsp32-antibody-161020241941452.jpg)
![Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77460] - HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77460-img0005.jpg "Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77460] - HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody [NBP1-77460] - Analysis of Heme Oxygenase 1 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-NBP1-77460-img0006.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Wild-type (WT) and HMOX1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-2120249374925.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Wild-type (WT) and HMOX1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-2120249393543.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects secreted Heme Oxygenase 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded cat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-212024940885.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-212024943288.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-2120249413392.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-2120249415928.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-2120249383177.jpg)
![HO-1/HMOX1/HSP32 Antibody (3L2U4) [NBP3-15640] - Analysis of extracts of HepG2 cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-3l2u4-western-blot-45202375028..jpg)
![<b>Genetic Strategies Validation. </b>HO-1/HMOX1/HSP32 Antibody (3L2U4) [NBP3-15640] -Analysis of extracts from wild type(WT) and Heme Oxygenase 1 (HO-1/HMOX1) knockout (KO) HeLa cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-3l2u4-knockout-validated-45202375233..jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (3L2U4) [NBP3-15640] -Analysis of human colon using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb at dilution of 1:200 (40x lens) .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-7h7w10-immunohistochemistry-paraffin-23720241236..jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (3L2U4) [NBP3-15640] -Human spleen using [KD Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-7h7w10-immunohistochemistry-paraffin-237202412427..jpg)
![Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77459-img0008.jpg "Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - Analysis of heme oxygenase mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-NBP1-77459-img0007.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining in human spleen and skeletal muscle tissues using NBP1-89964 antibody. Corresponding HMOX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining in human spleen and skeletal muscle tissues using NBP1-89964 antibody. Corresponding HMOX1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Analysis in human cell lines A-549 and MCF-7 using anti-HMOX1 antibody. Corresponding HMOX1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Western-Blot-NBP1-89964-img0016.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human Fallopian tube shows shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0018.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0019.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human skeletal muscle shows shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0020.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human spleen shows strong granular cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-89964-img0021.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Western blot analysis of HO-1/HMOX1/HSP32 on different lysates with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32420) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32420_rabbit-ho-1-hmox1-hsp32-mab-psh02-76-27820241524263.jpg "Western Blot: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Western blot analysis of HO-1/HMOX1/HSP32 on different lysates with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32420) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32420) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32420_rabbit-ho-1-hmox1-hsp32-mab-psh02-76-298202414262647.jpg)
![Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (PSH02-76) [NBP3-32420] - Immunocytochemistry analysis of A549 cells labeling HO-1/HMOX1/HSP32 with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HO-1/HMOX1/HSP32 antibody (NBP3-32420) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32420_rabbit-ho-1-hmox1-hsp32-mab-psh02-76-29202410484883.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0004.jpg "Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of Jurkat cells with HO-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Flow-Cytometry-NBP2-67382-img0001.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded human liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0002.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded human spleen tissue using anti-HO-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0003.jpg)