Novus Biologicals products are now on bio-techne.com

Recombinant Human Histone Deacetylase 2/HDAC2 His Protein

Images

 
SDS-Page: Recombinant Human Histone Deacetylase 2/HDAC2 Protein [NBP1-72399] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Recombinant Human Histone Deacetylase 2/HDAC2 His Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-488 of Human Histone Deacetylase 2/HDAC2

Source: Baculovirus

Amino Acid Sequence: MAYSQGGGKK KVCYYYDGDI GNYYYGQGHP MKPHRIRMTH NLLLNYGLYR KMEIYRPHKA TAEEMTKYHS DEYIKFLRSI RPDNMSEYSK QMQRFNVGED CPVFDGLFEF CQLSTGGSVA GAVKLNRQQT DMAVNWAGGL HHAKKSEASG FCYVNDIVLA ILELLKYHQR VLYIDIDIHH GDGVEEAFYT TDRVMTVSFH KYGEYFPGTG DLRDIGAGKG KYYAVNFPMR DGIDDESYGQ IFKPIISKVM EMYQPSAVVL QCGADSLSGD RLGCFNLTVK GHAKCVEVVK TFNLPLLMLG GGGYTIRNVA RCWTYETAVA LDCEIPNELP YNDYFEYFGP DFKLHISPSN MTNQNTPEYM EKIKQRLFEN LRMLPHAPGV QMQAIPEDAV HEDSGDEDGE DPDKRISIRA SDKRIACDEE FSDSEDEGEG GRRNVADHKK GAKKARIEED KKETEDKKTD VKEEDKSKDN SGEKTDTKGT KSEQLSNPSR HHHHHH

Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Gene
HDAC2
Purity
>85%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
56.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT, 0.1 mM PMSF
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human Histone Deacetylase 2/HDAC2 His Protein

  • EC 3.5.1.98
  • HD2
  • HDAC2
  • Histone Deacetylase 2
  • RPD3
  • transcriptional regulator homolog RPD3
  • YAF1
  • YY1-associated factor 1

Background

HDAC2 belongs to the histone deacetylase family that act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones. It forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. It also plays an important role in transcriptional regulation, cell cycle progression and developmental events. Recombinant human HDAC2 protein was expressed with c-terminal His-tag in high-5 cells using baculovirus expression system and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56340
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NBP2-03993
Species: Bv, Ca, Hu, Mu, Pm
Applications: WB
NB100-1669
Species: Hu
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP2-07996
Species: Hu
Applications: WB
NBP2-52486
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB500-171
Species: Hu, I, Mu, Rt, Xp, Ye
Applications: ChIP, IB, ICC/IF, IHC, IHC-P, Single-Cell Western, WB
H00007001-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
NLS3775
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
NBP1-85351
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00008294-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBL1-11570
Species: Hu
Applications: WB
H00008365-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA
NBL1-11567
Species: Hu
Applications: WB
AF5215
Species: Hu, Mu
Applications: ICC, WB
NBP2-22151
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
MAB2676
Species: Hu, Mu, Rt
Applications: ICC, WB
NBP1-72399
Species: Hu
Applications: PAGE

Publications for Histone Deacetylase 2/HDAC2 Recombinant Protein (NBP1-72399) (0)

There are no publications for Histone Deacetylase 2/HDAC2 Recombinant Protein (NBP1-72399).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Histone Deacetylase 2/HDAC2 Recombinant Protein (NBP1-72399) (0)

There are no reviews for Histone Deacetylase 2/HDAC2 Recombinant Protein (NBP1-72399). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Histone Deacetylase 2/HDAC2 Recombinant Protein (NBP1-72399). (Showing 1 - 1 of 1 FAQ).

  1. Does HDAC2 protein have deacetylase activity? Which substrate did you use?
    • NBP1-72399 was developed and has only been validated for use in SDS-PAGE. We have not evaluated the functional activity of this protein, and it's possible that it may not possess biological activity at all and we cannot guarantee it. It is suitable for use as a positive control in western blotting and potentially ELISA.

Additional Histone Deacetylase 2/HDAC2 Products

Research Areas for Histone Deacetylase 2/HDAC2 Recombinant Protein (NBP1-72399)

Find related products by research area.

Blogs on Histone Deacetylase 2/HDAC2

There are no specific blogs for Histone Deacetylase 2/HDAC2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

HDAC1 Antibody
NB100-56340

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Histone Deacetylase 2/HDAC2 His Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol HDAC2