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Histone Deacetylase 2/HDAC2 Antibody (OTI7E10)

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Western Blot: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC2.
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (7E10) [NBP2-45949] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI7E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 415-488 of human HDAC2(NP_001518) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HDAC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
55.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone Deacetylase 2/HDAC2 Antibody (OTI7E10)

  • EC 3.5.1.98
  • HD2
  • HDAC2
  • Histone Deacetylase 2
  • RPD3
  • transcriptional regulator homolog RPD3
  • YAF1
  • YY1-associated factor 1

Background

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HDAC2