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Histone Deacetylase 2/HDAC2 Antibody (806116)

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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, SH-SY5Y human neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, SH-SY5Y human neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse ...read more
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 98 kDa (as indicated) using ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western
Clone
806116
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB7679-SP
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Histone Deacetylase 2/HDAC2 Antibody (806116) Summary

Immunogen
E. coli-derived recombinant human Histone Deacetylase 2/HDAC-2
Pro386-Pro488
Accession # Q92769
Specificity
Detects human Histone Deacetylase 2/HDAC-2 in direct ELISAs and human and rat Histone Deacetylase 2/HDAC-2 Western blots. In direct ELISAs, no cross-reactivity with recombinant human HDAC1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HDAC2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using MAB7679.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Histone Deacetylase 2/HDAC2 Antibody (806116)

  • EC 3.5.1.98
  • HD2
  • HDAC2
  • Histone Deacetylase 2
  • RPD3
  • transcriptional regulator homolog RPD3
  • YAF1
  • YY1-associated factor 1

Background

Histone deacetylase 2 (HDAC2), is a ubiquitously expressed intracellular enzyme that is involved in the post-translational modification of proteins. Acetyl groups are added to e-NH3 on lysine residues by acetyltransferases and removed by HDACs. The balance between these reactions has widespread effects on the enzymatic activity, subcellular localization, and molecular interactions of the substrates. HDAC2 can associate into several multiprotein complexes with discrete functions. Acetylation of core histones (H2A, H2B, H3, and H4) alters their interaction with DNA and the local availability of DNA for transcription. The function of HDAC2 itself is regulated by several post-translational modifications including phosphorylation, acetylation, ubiquitination, tyrosine nitrosylation, and cysteine carbonylation. Within aa 386-488, human HDAC2 shares 98% and 96% aa sequence identity with mouse and rat HDAC2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Histone Deacetylase 2/HDAC2 Antibody (806116)
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Publications for Histone Deacetylase 2/HDAC2 Antibody (MAB7679)(2)


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Bioinformatics

Gene Symbol HDAC2
Uniprot