![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Western blot analysis of extracts of various cell lines, using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-10R7O1-Western-Blot-NBP3-15824-img0006.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Western blot analysis of extracts of various cell lines, using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IP |
| Clone | 10R7O1 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone Deacetylase 2/HDAC2 (NP_001518.3). MAYSQGGGKKKVCYYYDGDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKATAEEMTKYHSDEYIKFLRSIRPDNMSEYSKQMQRFNVGED |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HDAC2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.05% BSA, 50% glycerol, pH7.3 |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunocytochemistry/ Immunofluorescence Histone H4 [ac Lys5] Antibody - BSA Free](https://images.novusbio.com/images/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation Histone H4 [ac Lys5] Antibody - BSA Free](https://images.novusbio.com/images/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Western Blot Histone H4 [ac Lys5] Antibody - BSA Free](https://images.novusbio.com/images/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0009.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone Deacetylase 2/HDAC2 Antibody (NBP3-15824)Find related products by research area.
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| Gene Symbol | HDAC2 |
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunofluorescence analysis of NIH-3T3 cells using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-10R7O1-Immunocytochemistry-Immunofluorescence-NBP3-15824-img0005.jpg "Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunofluorescence analysis of NIH-3T3 cells using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunohistochemistry of paraffin-embedded mouse brain using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-10R7O1-Immunohistochemistry-Paraffin-NBP3-15824-img0003.jpg "Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunohistochemistry of paraffin-embedded mouse brain using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunofluorescence analysis of C6 cells using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-10R7O1-Immunocytochemistry-Immunofluorescence-NBP3-15824-img0004.jpg "Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunofluorescence analysis of C6 cells using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunohistochemistry of paraffin-embedded human oophoroma using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-10R7O1-Immunohistochemistry-Paraffin-NBP3-15824-img0001.jpg "Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunohistochemistry of paraffin-embedded human oophoroma using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunohistochemistry of paraffin-embedded rat spleen using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-10R7O1-Immunohistochemistry-Paraffin-NBP3-15824-img0002.jpg "Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunohistochemistry of paraffin-embedded rat spleen using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunoprecipitation: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Histone Deacetylase 2/HDAC2 antibody (NBP3-15824). Western blot was performed from the immunoprecipitate using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-10R7O1-Immunoprecipitation-NBP3-15824-img0007.jpg "Immunoprecipitation: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Histone Deacetylase 2/HDAC2 antibody (NBP3-15824). Western blot was performed from the immunoprecipitate using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at a dilition of 1:1000.")
![Western Blot CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/CBP_MAB2676_Western_Blot_6043.jpg)
![Immunocytochemistry CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/mab2676_human-mouse-rat-cbp-mab-clone-257003-immunocytochemistry-101220208237.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")